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Mini-Reviews in Organic Chemistry

Editor-in-Chief

ISSN (Print): 1570-193X
ISSN (Online): 1875-6298

Peptide Arrays for the Analysis of Antibody Epitope Recognition Patterns

Author(s): Ulf Reimer, Ulrich Reineke and Mike Schutkowski

Volume 8 , Issue 2 , 2011

Page: [137 - 146] Pages: 10

DOI: 10.2174/157019311795177835

Price: $65

Abstract

One of the first applications of peptide arrays in general and those prepared by SPOT synthesis in particular was the mapping of antibody epitopes. In this article the diverse applications in this field are described. Different types of peptide libraries such as peptide scans, substitution analyses, truncation and deletion libraries as well as combinatorial and randomly generated libraries are briefly covered. Furthermore, their applications for antibody epitope mapping are described. These are: (i) identification of antigenic determinants within protein sequences for monoclonal and polyclonal antibodies, (ii) identification of key residues for binding, mapping of linear and discontinuous binding sites, (iii) paratope mapping, (iv) identification of mimotopes, and finally (v) the profiling of complex (auto)antibody signatures in biological fluids such as human or mice sera to identify novel biomarkers for e.g. cancer, allergy, infectious and autoimmune diseases.

Keywords: Antibody, antigen, diagnostics, epitope mapping, peptide library, peptide microarray, peptide scan, SPOT synthesis


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