Expression, Purification, Crystallization and Preliminary X-Ray Crystallographic Analysis of the Resuscitation Promoting Factor Interacting Protein RipA from M. tuberculosis

Title: Expression, Purification, Crystallization and Preliminary X-Ray Crystallographic Analysis of the Resuscitation Promoting Factor Interacting Protein RipA from M. tuberculosis

Volume: 17 Issue: 1

Author(s): Alessia Ruggiero, Flavia Squeglia, Carla Esposito, Daniela Marasco, Emilia Pedone, Carlo Pedone and Rita Berisio

Affiliation:

Keywords: Resuscitation promoting factor, crystal, growth factor, x-ray, tuberculosis

Abstract: RipA is an important growth factor of M. tuberculosis. Its depletion produces decreasing bacterial growth and abnormal phenotype. RipA C-terminal fragment (263-472), containing its predicted catalytic domain has been successfully crystallized using vapor-diffusion methods. The structure has been solved by Multiwavelength Anomalous Dispersion and atomic resolution refinement is in progress.

Cite this article as:

Ruggiero Alessia, Squeglia Flavia, Esposito Carla, Marasco Daniela, Pedone Emilia, Pedone Carlo and Berisio Rita, Expression, Purification, Crystallization and Preliminary X-Ray Crystallographic Analysis of the Resuscitation Promoting Factor Interacting Protein RipA from M. tuberculosis, Protein & Peptide Letters 2010; 17 (1) . https://dx.doi.org/10.2174/092986610789909557