Efficient Manual Fmoc Solid-Phase Synthesis of the N-Terminal Segment of Surfactant Protein B (SP-B1-25)

Title: Efficient Manual Fmoc Solid-Phase Synthesis of the N-Terminal Segment of Surfactant Protein B (SP-B1-25)

Volume: 16 Issue: 7

Author(s): Suria Ramli, Ian R. Gentle and Benjamin P. Ross

Affiliation:

Keywords: Surfactant protein B (SP-B), Fmoc solid-phase peptide synthesis, peptide aggregation, low-loading resin, methionine sulfoxide, tetrabutylammonium bromide

Abstract: The N-terminal 25 residue segment of human surfactant protein B (SP-B1-25) was synthesised in 26% yield by manual Fmoc solid-phase peptide synthesis (Fmoc SPPS) using low-loading Fmoc-Gly-Wang resin. Substantial oxidation of Met21 occurred during the synthesis, and the addition of Bu4NBr to a TFA/water/EDT/TIS cleavage cocktail enabled facile reduction of Met(O)21-SP-B1-25 to SP-B1-25. The methods described herein are generally applicable to the Fmoc SPPS of difficult sequences containing methionine.

Cite this article as:

Ramli Suria, Gentle R. Ian and Ross P. Benjamin, Efficient Manual Fmoc Solid-Phase Synthesis of the N-Terminal Segment of Surfactant Protein B (SP-B1-25), Protein & Peptide Letters 2009; 16 (7) . https://dx.doi.org/10.2174/092986609788681706