Efficient Production of Human β-Defensin 2 (HBD2) in Escherichia coli

Title: Efficient Production of Human β-Defensin 2 (HBD2) in Escherichia coli

Volume: 16 Issue: 6

Author(s): Thomas Vargues, Gareth J. Morrison, Emily S. Seo, David J. Clarke, Helen L. Fielder, Julien Bennani, Uday Pathania, Fiona Kilanowski, Julia R. Dorin, John R. W. Govan, C. Logan Mackay, Dusan Uhrin and Dominic J. Campopiano

Affiliation:

Keywords: HBD2, Defensin, Peptide, E. coli, Chemotaxis, Antimicrobial activity

Abstract: Human β-defensin 2 (HBD2) has been shown to interact with pathogenic bacteria and components of the mammalian innate and adaptive immune response. We describe a quick and reliable method for the production of HBD2 in Escherichia coli. HBD2 was expressed as an insoluble fusion, chemically cleaved and oxidised to give a single, folded HBD2 β-isoform. The purified peptide was analysed by high resolution mass spectrometry, displayed a well-dispersed H NMR spectrum, was a chemoattractant to HEK293 cells expressing CCR6 and acted as an antimicrobial agent against E. coli, P. aeruginosa, C. albicans and S. aureus.

Cite this article as:

Vargues Thomas, Morrison J. Gareth, Seo S. Emily, Clarke J. David, Fielder L. Helen, Bennani Julien, Pathania Uday, Kilanowski Fiona, Dorin R. Julia, Govan R. W. John, Mackay Logan C., Uhrin Dusan and Campopiano J. Dominic, Efficient Production of Human β-Defensin 2 (HBD2) in Escherichia coli, Protein & Peptide Letters 2009; 16 (6) . https://dx.doi.org/10.2174/092986609788490122