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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Pomegranin, An Antifungal Peptide from Pomegranate Peels

Author(s): Guang Guo, He Xiang Wang and Tzi Bun Ng

Volume 16, Issue 1, 2009

Page: [82 - 85] Pages: 4

DOI: 10.2174/092986609787049330

Price: $65


A new antifungal peptide designated as pomegranin, with an N-terminal sequence resembling that of rice disease resistance NB-S-LRR-like protein, was isolated from fresh pomegranate peels by ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, and gel filtration by fast protein liquid chromatography on Superdex 75. Pomegranin was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel blue gel. It exhibited a molecular mass of 11 kDa in both gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It inhibited mycelial growth in the fungi Botrytis cinerea and Fusarium oxysporum with an IC50 of 2 μM and 6.1 μM, respectively. It was devoid of hemagglutinating, ribonuclease, deoxyribonuclease and protease inhibitory activities.

Keywords: antifungal peptide, pomegranate, isolation, peel

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