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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Crystallization and Preliminary Crystallographic Analysis of Laminarinase from Rhodothermus marinus: A Case of Pseudomerohedral Twinning

Author(s): Alexander M. Golubev, Adriana L. Rojas, Alessandro S. Nascimento, Lucas Bleicher, Anna A. Kulminskaya, Elena V. Eneyskaya and Igor Polikarpov

Volume 15, Issue 10, 2008

Page: [1142 - 1144] Pages: 3

DOI: 10.2174/092986608786071139

Price: $65


Thermophilic endo-1,3(4)-β-glucanase (laminarinase) from Rhodothermus marinus was crystallized by the hanging-drop vapor diffusion method. The needle-like crystals belong to space group P21 and contain two protein molecules in the asymmetric unit with a solvent content of 51.75 %. Diffraction data were collected to a resolution of 1.95Å and resulted in a dataset with an overall Rmerge of 10.4% and a completeness of 97.8%. Analysis of the structure factors revealed pseudomerohedral twinning of the crystals with a twin fraction of approximately 42%.

Keywords: Laminarinase, Rhodothermus marinus, crystallization, pseudomerohedral twinning

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