Co-Expression of GroEL/ES Enhances the Expression of Plant Catalase in Bacterial Cytosol

Title: Co-Expression of GroEL/ES Enhances the Expression of Plant Catalase in Bacterial Cytosol

Volume: 15 Issue: 10

Author(s): Prosenjit Mondal, Mamata Ray, Sushmita Sahu and Surendra Chandra Sabat

Affiliation:

Keywords: GroEL/ES, Soluble expression, western blotting, normal and variant rice catalase-B, Plant protein, Heterologous expression

Abstract: Expression of plant proteins in E. coli is frequently unsuccessful, but soluble and functional rice catalase-B can be produced in E. coli when it is co-expressed with the chaperone GroEL/ES. The rice catalase exhibited properties typical for a catalase including the decomposition of H2O2 and inhibition by aminotriazole, a specific inhibitor for plant and animal catalases. This achievement records for first time the successful expression of a both native and variant rice plant catalase in bacterial cytosol suggesting that it may be an option to be considered for the expression of other plant proteins in E. coli.

Cite this article as:

Mondal Prosenjit, Ray Mamata, Sahu Sushmita and Sabat Chandra Surendra, Co-Expression of GroEL/ES Enhances the Expression of Plant Catalase in Bacterial Cytosol, Protein & Peptide Letters 2008; 15(10) . https://dx.doi.org/10.2174/092986608786071058