Synthesis of Acyl Carrier Protein Using a New Heterocyclic Carboxyl Activating Group, 3-Mercapto-5,6-Diphenyl-1,2,4-Triazine Through SPPS Strategy

Title: Synthesis of Acyl Carrier Protein Using a New Heterocyclic Carboxyl Activating Group, 3-Mercapto-5,6-Diphenyl-1,2,4-Triazine Through SPPS Strategy

Volume: 15 Issue: 10

Author(s): M. P. Rajan and E. Purushothaman

Affiliation:

Keywords: Acyl Carrier Protein, Synthesis, SPPS Strategy, carboxyl activating group, UV- visible spectra, mercapto heterocycle, peptide synthesis, CLPSER resin, HPLC

Abstract: A new, effective and easily accessible carboxyl activating group , 3-mercapto-5, 6 - diphenyl-1, 2, 4 - triazine was developed and the effectiveness was investigated using acyl derivatives, chemically by the preparation of respective amides, in addition to monitoring spectrophotometrically exploiting the UV- visible spectra. The carboxyl activating nature of the above mercapto heterocycle was further confirmed by solid phase peptide synthesis. Here CLPSER resin as polymeric support was applied. We synthesized Acyl Carrier Protein and smaller peptides by replacing this thiol for other conventional carboxyl activating reagents. These peptides synthesized were purified by HPLC and characterized by molecular weight method.

Cite this article as:

Rajan P. M. and Purushothaman E., Synthesis of Acyl Carrier Protein Using a New Heterocyclic Carboxyl Activating Group, 3-Mercapto-5,6-Diphenyl-1,2,4-Triazine Through SPPS Strategy, Protein & Peptide Letters 2008; 15 (10) . https://dx.doi.org/10.2174/092986608786071148