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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Comparative Studies on the Aggregation Behavior of HBPs from Human Seminal Plasma by Dynamic Light Scattering

Author(s): Vijay Kumar, Md. Imtaiyaz Hassan, Tej P. Singh and Savita Yadav

Volume 15, Issue 6, 2008

Page: [633 - 639] Pages: 7

DOI: 10.2174/092986608784966903

Price: $65


Heparin binding proteins (HBPs) from human seminal plasma (HSP) were obtained by heparin affinity and size exclusion chromatography. The aggregation/disaggregation of HBPs was followed by dynamic light scattering (DLS) in presence of various physiological ligands such as CaCl2, NaCl, EDTA, cholesterol, adenosine, D-glucose and D-fructose. The aggregation pattern of lactoferrin was also analyzed and compared. The study of interactions of HBPs of HSP may contribute to an understanding mechanisms underlying the fertilization process.

Keywords: Protein-protein aggregation, chromatography, dynamic light scattering, HBPs, HSP, sperm capacitation

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