Protein Preparation, Crystallization and Preliminary X-Ray Crystallographic Analysis of N-Acetylglutamate Kinase from Streptococcus mutans

Title: Protein Preparation, Crystallization and Preliminary X-Ray Crystallographic Analysis of N-Acetylglutamate Kinase from Streptococcus mutans

Volume: 15 Issue: 5

Author(s): Chang-Wei Wu, Lan-Fen Li, Xiang Liu, Xiong-Zhuo Gao, Jian Lei, Xiao-Dong Su, Xiaojun Zhao and Yu-He Liang

Affiliation:

Keywords: Streptococcus mutans, N-acetylglutamate kinase (NAGK), arginine biosynthesis, protein crystallography

Abstract: The N-acetylglutamate kinase from Streptococcus mutans was expressed in Escherichia coli in soluble form and purified to homogeneity. Crystals suitable for X-ray diffraction were obtained by hanging-drop vapor diffusion method and diffracted to 2.06 Å. The crystal belonged to space group P21212, with unit cell parameters a = 57.19 Å, b =94.76 Å, c =47.58 Å. The gel filtration and initial phasing results showed that the enzyme exists as a monomer, which is different from previously reported N-acetylglutamate kinases.

Cite this article as:

Wu Chang-Wei, Li Lan-Fen, Liu Xiang, Gao Xiong-Zhuo, Lei Jian, Su Xiao-Dong, Zhao Xiaojun and Liang Yu-He, Protein Preparation, Crystallization and Preliminary X-Ray Crystallographic Analysis of N-Acetylglutamate Kinase from Streptococcus mutans, Protein & Peptide Letters 2008; 15(5) . https://dx.doi.org/10.2174/092986608784567519