Abstract
AxCesD protein required for bacterial cellulose biosynthesis in Acetobacter xylinum was overexpressed in E. coli, purified and crystallized. Single crystals of SeMet-substituted AxCesD were obtained by the sitting-drop vapordiffusion method. The crystal belongs to the primitive trigonal space group P32, with unit-cell parameters a = b = 77.7 Å, and c = 213.9 Å. The asymmetric unit in the crystal was assumed to contain 8 protein molecules giving the Matthews coefficient (VM ) of 2.54 Å3 Da-1. Se-MAD data were collected to 2.3 Å resolution using synchrotron radiations.
Keywords: AxCesD, bacterial cellulose, biosynthesis, crystal, Acetobacter xylinum
Protein & Peptide Letters
Title: Purification, Crystallization and Preliminary X-Ray Studies of AxCesD Required for Efficient Cellulose Biosynthesis in Acetobacter xylinum
Volume: 15 Issue: 1
Author(s): Min Yao, SongQing Hu, YongGui Gao, Kenji Tajima, Takanori Yoda, Daisuke Shimura, Yasuharu Satoh, Shin Kawano, Isao Tanaka and Masanobu Munekata
Affiliation:
Keywords: AxCesD, bacterial cellulose, biosynthesis, crystal, Acetobacter xylinum
Abstract: AxCesD protein required for bacterial cellulose biosynthesis in Acetobacter xylinum was overexpressed in E. coli, purified and crystallized. Single crystals of SeMet-substituted AxCesD were obtained by the sitting-drop vapordiffusion method. The crystal belongs to the primitive trigonal space group P32, with unit-cell parameters a = b = 77.7 Å, and c = 213.9 Å. The asymmetric unit in the crystal was assumed to contain 8 protein molecules giving the Matthews coefficient (VM ) of 2.54 Å3 Da-1. Se-MAD data were collected to 2.3 Å resolution using synchrotron radiations.
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Cite this article as:
Yao Min, Hu SongQing, Gao YongGui, Tajima Kenji, Yoda Takanori, Shimura Daisuke, Satoh Yasuharu, Kawano Shin, Tanaka Isao and Munekata Masanobu, Purification, Crystallization and Preliminary X-Ray Studies of AxCesD Required for Efficient Cellulose Biosynthesis in Acetobacter xylinum, Protein & Peptide Letters 2008; 15 (1) . https://dx.doi.org/10.2174/092986608783330422
DOI https://dx.doi.org/10.2174/092986608783330422 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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