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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Cdna Cloning And Expression Of Acutin Ii, A Native Mutant Of Thrombin-Like Enzyme From Agkistrodon Acutus

Author(s): Hua Pan, Jiewu Liu, Xiaoyan Du, Yuancong Zhou and Xiangfu Wu

Volume 8, Issue 6, 2001

Page: [469 - 475] Pages: 7

DOI: 10.2174/0929866013409139

Price: $65


One cDNA of a thrombin-like enzyme named acutinII was amplified by RT-PCR from the venom gland of the Agkistrodon acutus snake. AcutinII shows high similarity to acutin, the other thrombin-like enzyme from Agkistrodon acutus snake in nucleotide and amino acid sequences except it is shorter by 16 amino acid at the C-terminus. The catalytic residues and disulfide bridges of acutinII were deduced. AcutinII was expressed in E. coli .The expressed protein was detected by western blotting and the yield was up to 32 percent of the total proteins.

Keywords: CDNA CLONING, ACUTIN II, AGKISTRODON ACUTUS, Snake venom, Thrombin-like enzyme, RT-PCR, Expression, thrombosis

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