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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Cloning and Expression of a Novel Long Neurotoxin from Bungarus multicinctus in E. coli

Author(s): Qin Cai, Sheng-li Yang and Yi Gong

Volume 8, Issue 3, 2001

Page: [201 - 207] Pages: 7

DOI: 10.2174/0929866013409508

Price: $65


The total RNA was isolated from Bungarus multicinctus venom. A novel long neurotoxin (LNT) cDNA was amplified by RT-PCR method. Sequence analysis revealed that the cDNA of long neurotoxin has an open reading frame encoding 94 amino acids, including a signal peptide of 21 amino acids. The mature peptide encoding sequence was inserted into plasmid pET-28 (a+), and then which was expressed in the E. coli BL21 (DE3). The expressed Protein is identified as a novel long neurotoxin by western blot.

Keywords: NEUROTOXIN, total RNA, neurotoxin (LNT) cDNA

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