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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

One-Step Affinity Purification of Human Mu-Opioid Receptor Overexpressed in Baculovirus System

Author(s): Chen Li. Wei, Feng Ya. Ping, Zhou De He, Xu xue-Jun, Chen Jie, Wei Qiang and Chi Zhi-Qiang

Volume 8, Issue 4, 2001

Page: [265 - 272] Pages: 8

DOI: 10.2174/0929866013409373

Price: $65

Abstract

Affinity chromatography on Ni-nitrilotriacetic acid agarose was used to purify human mu-opioid receptors overexpressed in Sf9 cells. We introduced a tag of 6 consecutive histidines at its carboxyl terminus for easy purification of recombinant proteins. The binding activity and identification of the purified receptor were determined by receptor binding assay, SDS-PAGE and Western blotting analysis. This procedure used in the paper offer an easy and fast route to the purification of recombinant human mu-opioid receptors.

Keywords: BACULOVIRUS, opioid receptor (MOR), Spodoptera frugiperda, Trichoplusia ni, Receptor binding assay


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