Production of Recombinant Human Calcitonin from Silkworm (B. Mori) Larvae infected by Baculovirus

Guan-zhen      Yang; Zhen-zhen      Chen; Da-fu      Cui; Bo-liang      Li; Xiang-fu      Wu

doi:10.2174/0929866023408689

Abstract

A synthetic modified gene encoding the human calcitonin analog (hmCT) was expressed by use of the baculovirus expression system. After injection with recombinant baculoviruses, the hmCT-GST fusion protein was produced within the silkworm larvae. The fusion protein was purified by affinity chromatography. Biological activity of hmCT for hypercalcemic effect was determined in normal rats.

Keywords: Human Calcitonin, hmCT-GST fusion, recombinant baculoviruses, silkworm larvae, hypercalcemic effect

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Protein & Peptide Letters

Title: Production of Recombinant Human Calcitonin from Silkworm (B. Mori) Larvae infected by Baculovirus

Volume: 9 Issue: 4

Author(s): Guan-zhen Yang, Zhen-zhen Chen, Da-fu Cui, Bo-liang Li and Xiang-fu Wu

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Keywords: Human Calcitonin, hmCT-GST fusion, recombinant baculoviruses, silkworm larvae, hypercalcemic effect

Abstract: A synthetic modified gene encoding the human calcitonin analog (hmCT) was expressed by use of the baculovirus expression system. After injection with recombinant baculoviruses, the hmCT-GST fusion protein was produced within the silkworm larvae. The fusion protein was purified by affinity chromatography. Biological activity of hmCT for hypercalcemic effect was determined in normal rats.

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Yang Guan-zhen, Chen Zhen-zhen, Cui Da-fu, Li Bo-liang and Wu Xiang-fu, Production of Recombinant Human Calcitonin from Silkworm (B. Mori) Larvae infected by Baculovirus, Protein & Peptide Letters 2002; 9 (4) . https://dx.doi.org/10.2174/0929866023408689

DOI https://dx.doi.org/10.2174/0929866023408689	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305

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