TAT-Liposomes: A Novel Intracellular Drug Carrier

Title: TAT-Liposomes: A Novel Intracellular Drug Carrier

Volume: 4 Issue: 2

Author(s): V. P. Torchilin and T. S. Levchenko

Affiliation:

Keywords: TAT-Liposomes, peptide, PEGylated, liposomes, non-endocytotic

Abstract: TAT peptide was attached to the surface of plain and PEGylated liposomes. These TAT peptide-modified liposomes have been shown to translocate into a variety of normal and cancer cells if a non-hindered interaction between the cell surface and liposome-attached TAT peptide was made possible. TAT peptide-liposomes translocated into cells remain intact within first few hours as proved by a co-localization of fluorescent markers entrapped inside liposomes and incorporated into the liposomal membrane. After 2 hours liposomes had slowly migrating towards cell nuclei. Liposomes had completely disintegrated with their inner marker released by approximately 9 hours. TAT peptide-liposomes were made slightly cationic by adding up to 10 mol % of a cationic lipid (DOTAP). These slightly cationic liposomes were non-toxic towards cells, formed firm complexes with DNA (plasmid encoding for the formation of the Green Fluorescent Protein), and efficiently transfected a variety of cells. TAT peptideliposomes can be considered as promising carriers for the non-endocytotic intracellular delivery of drugs and DNA.

Cite this article as:

Torchilin P. V. and Levchenko S. T., TAT-Liposomes: A Novel Intracellular Drug Carrier, Current Protein & Peptide Science 2003; 4 (2) . https://dx.doi.org/10.2174/1389203033487298