Crystallization And Preliminary X-Ray Diffraction Study Of Hemoglobin D From The Aldabra Giant Tortoise, Geochelone Gigantea

Title: Crystallization And Preliminary X-Ray Diffraction Study Of Hemoglobin D From The Aldabra Giant Tortoise, Geochelone Gigantea

Volume: 10 Issue: 4

Author(s): Takao Kuwada, Tomokazu Hasegawa, Isamu Satoh, Koichi Ishikawa and Fumio Shishikura

Affiliation:

Keywords: hemoglobin d, crystallization, x-ray diffraction, reptilia, the aldabra giant tortoise, geochelone gigantea

Abstract: Hemoglobin D (Hb D) from the Aldabra giant tortoise, Geochelone gigantea, was crystallized by the hanging drop vapor diffusion technique with a precipitant solution containing 10% polyethylene glycol 3350 and 50 mM HEPES-Na, pH 7.5. The Hb D crystals of G. gigantea, which diffract to at least a 2.0 Å resolution, belong to the monoclinic space group C2 with unit cell dimensions of a = 112.1 Å, b = 62.4 Å, c = 54.0 Å, and β = 110.3 . One αβ dimer molecule of Hb D existed in an asymmetric unit, with a calculated value of Vm of 2.77 Å3Da-1.

Cite this article as:

Kuwada Takao, Hasegawa Tomokazu, Satoh Isamu, Ishikawa Koichi and Shishikura Fumio, Crystallization And Preliminary X-Ray Diffraction Study Of Hemoglobin D From The Aldabra Giant Tortoise, Geochelone Gigantea, Protein & Peptide Letters 2003; 10 (4) . https://dx.doi.org/10.2174/0929866033478799