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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Crystallization And Preliminary X-Ray Diffraction Study Of Hemoglobin D From The Aldabra Giant Tortoise, Geochelone Gigantea

Author(s): Takao Kuwada, Tomokazu Hasegawa, Isamu Satoh, Koichi Ishikawa and Fumio Shishikura

Volume 10, Issue 4, 2003

Page: [422 - 425] Pages: 4

DOI: 10.2174/0929866033478799

Price: $65


Hemoglobin D (Hb D) from the Aldabra giant tortoise, Geochelone gigantea, was crystallized by the hanging drop vapor diffusion technique with a precipitant solution containing 10% polyethylene glycol 3350 and 50 mM HEPES-Na, pH 7.5. The Hb D crystals of G. gigantea, which diffract to at least a 2.0 Å resolution, belong to the monoclinic space group C2 with unit cell dimensions of a = 112.1 Å, b = 62.4 Å, c = 54.0 Å, and β = 110.3 . One αβ dimer molecule of Hb D existed in an asymmetric unit, with a calculated value of Vm of 2.77 Å3Da-1.

Keywords: hemoglobin d, crystallization, x-ray diffraction, reptilia, the aldabra giant tortoise, geochelone gigantea

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