New Chemical Crosslinking Methods for the Identification of Transient Protein-Protein Interactions with Multiprotein Complexes

Title: New Chemical Crosslinking Methods for the Identification of Transient Protein-Protein Interactions with Multiprotein Complexes

Volume: 5 Issue: 4

Author(s): K. Melcher

Affiliation:

Keywords: multiprotein complexes, photoactivatable crosslinking, label transfer

Abstract: Most proteins function as multiprotein complexes or interact with multiprotein complexes. Identification of protein-protein interactions in the context of their physiologically relevant complexes is therefore key to fully understand the cellular machinery. Here I discuss advances in chemical crosslinking methods that allow investigators to map direct subunit contacts in transient interactions with multimeric complexes. Methods discussed fall into two categories: (i) in vitro approaches with localized, inducible crosslinking reagents and (ii) in vivo approaches with unlocalized crosslinkers.

Cite this article as:

Melcher K., New Chemical Crosslinking Methods for the Identification of Transient Protein-Protein Interactions with Multiprotein Complexes, Current Protein & Peptide Science 2004; 5 (4) . https://dx.doi.org/10.2174/1389203043379701