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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

α-Amylase Inhibitors from Ficus Sp. Seeds and Their Activities Towards Coleoptera Insect Pests

Author(s): Ingrid W.L Bezerra, Fabiano M. Teixeira, Adeliana S. Oliveira, Carina L. Araujo, Edda L. Leite, Karla C.S. Queiroz and Mauricio P. Sales

Volume 11, Issue 2, 2004

Page: [181 - 187] Pages: 7

DOI: 10.2174/0929866043478329

Price: $65

Abstract

α-Amylase Inhibitors were isolated from Ficus sp. (Gameleira) seeds by acetone fractionation and Sephadex G-50. Two inhibitors (α-PPAI and α-ZSAI) were tested against α-amylases from coleopteran larvae. α-PPAI was active to α-amylases of Callosobruchus maculatus (52%) and Zabrotes subfasciatus (53%). α-ZSAI was strongly active to Z. subfasciatus (100%) of and Mimosestes mimosae (98%). The α-ZSAI is a glycoprotein of ∼50kDa with an IC50 value of 0.074 μg μl-1.

Keywords: Ficus sp,, Zabrotes subfasciatus,, Callosobruchus macutatus,, Mimosestes mimosae,


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