Abstract
Calcitonin (CT) is a peptide hormone produced by the parafollicular cells of the thyroid gland in mammals and by the ultimobranchial gland of birds and fish. Salmon calcitonin (sCT), which is more potent and longer lasting than human CT, has been used widely for the treatment of osteoporosis, pagets disease, hypercalcemic shock and chronic pain in terminal cancer patients. sCT is one of the many bioactive peptides that require C-terminal amidation for full biological activity. In this study we describe the overexpression and over-production of C-terminal amidated sCT in recombinant Streptomyces avermitilis. With this approach the utilization of expensive peptide synthesis can be circumvented.
Keywords: salmon calcitonin, amidating enzyme, Streptomyces avermitilis,, plasmid ChiC9.
Protein & Peptide Letters
Title: Overexpression, Purification and Characterization of Recombinant Salmon Calcitonin, A Therapeutic Protein, in Streptomyces Avermitilis
Volume: 11 Issue: 2
Author(s): Chiranjib Chakraborty, Shyam Nandi and S. Sinha
Affiliation:
Keywords: salmon calcitonin, amidating enzyme, Streptomyces avermitilis,, plasmid ChiC9.
Abstract: Calcitonin (CT) is a peptide hormone produced by the parafollicular cells of the thyroid gland in mammals and by the ultimobranchial gland of birds and fish. Salmon calcitonin (sCT), which is more potent and longer lasting than human CT, has been used widely for the treatment of osteoporosis, pagets disease, hypercalcemic shock and chronic pain in terminal cancer patients. sCT is one of the many bioactive peptides that require C-terminal amidation for full biological activity. In this study we describe the overexpression and over-production of C-terminal amidated sCT in recombinant Streptomyces avermitilis. With this approach the utilization of expensive peptide synthesis can be circumvented.
Export Options
About this article
Cite this article as:
Chakraborty Chiranjib, Nandi Shyam and Sinha S., Overexpression, Purification and Characterization of Recombinant Salmon Calcitonin, A Therapeutic Protein, in Streptomyces Avermitilis, Protein & Peptide Letters 2004; 11 (2) . https://dx.doi.org/10.2174/0929866043478266
DOI https://dx.doi.org/10.2174/0929866043478266 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Human Hepatocytes as a Tool for Studying Toxicity and Drug Metabolism
Current Drug Metabolism Emerging Toxicity Aspects of Silver Nanoparticles: An Overview
Current Nanomaterials Biosynthesis of Silver Nanoparticles from Synechocystis sp to be Used as a Flocculant Agent with Different Microalgae Strains
Current Nanomaterials QSPR in Oral Bioavailability: Specificity or Integrality?
Mini-Reviews in Medicinal Chemistry Silybin and Silymarin - New and Emerging Applications in Medicine
Current Medicinal Chemistry Nasal Drug Delivery System-Factors Affecting and Applications
Current Drug Therapy Clinical Uses of Melatonin: Evaluation of Human Trials
Current Medicinal Chemistry Computer-Assisted Methods in Chemical Toxicity Prediction
Mini-Reviews in Medicinal Chemistry New Anthocyanins from Black Elderberry of Inhibitory Potential Revealed by Mass Spectrometry
The Natural Products Journal Nanomedicine
Current Nanoscience Protein-protein Interactions: Network Analysis and Applications in Drug Discovery
Current Pharmaceutical Design New Pharmacological Perspectives and Therapeutic Potential of PPAR-γ Agonists
Current Pharmaceutical Design Rectal Cancer: Adjuvant Therapy and New Directions
Reviews on Recent Clinical Trials An Update of the Classical and Novel Methods Used for Measuring Fast Neurotransmitters During Normal and Brain Altered Function
Current Neuropharmacology Calreticulin is Differentially Expressed in Invasive Ductal Carcinoma: A Comparative Study
Current Proteomics Thymidylate Kinase: An Old Topic Brings New Perspectives
Current Medicinal Chemistry Specific Targeting of HER2-Positive Head and Neck Squamous Cell Carcinoma Line HN5 by Idarubicin-ZHER2 Affibody Conjugate
Current Cancer Drug Targets Design, Synthesis and Biological Evaluation of 2, 4, 5-Triphenylimidazole Derivatives with Preliminary SAR
Letters in Drug Design & Discovery Versatile Synthesis of Organic Compounds Derived from Ascorbic Acid
Current Organocatalysis Carotenoids in Photooxidative Stress
Current Nutrition & Food Science