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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Fluorescent Modification Of N-Terminal Amino Group In Peptides For Complete Sequence Analysis Using Psd Method In Maldi-Tof-Ms

Author(s): Masatoshi Nakagawa and Hiroshi Nakanishi

Volume 11 , Issue 1 , 2004

Page: [71 - 77] Pages: 7

DOI: 10.2174/0929866043478464

Price: $65

Abstract

In the sequence analyses of peptides from nucleoprotein in influenza virus, it was very difficult to obtain the sufficient numbers of fragment ions using the post-source decay (PSD) method in MALDITOF- MS. Fluorescent modification of the N-terminal amino group in the target peptides was introduced for the PSD measurement. The fluorescently-labelled peptides gave sufficient fragment ions in the PSD spectrum, which leads to the complete sequence analysis of peptide.

Keywords: maldi-tof-ms, psd method, peptide sequence, fluorescently modification, nucleoprotein in influenza virus


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