Seed Protein Variation Among Pepper (Capsicum Sp.) Genotypes Revealed By Maldi-Tof Analysis

Marco   Aurelio   Caldas de Pinho Pessoa Filho; Carlos   Bloch   Junior; Danilo   Fernandes da Silva   Filho; Alexsandro   Sobreira   Galdino; Rodrigo   Maranguape Silva da   Cunha; Maria   Aparecida Oliveira   Alves; Thalles   Barbosa   Grangeiro

doi:10.2174/0929866043478482

Abstract

A method for seed proteome analysis using MALDI-TOF mass spectrometry is described. The data were used to estimate the genetic diversity degree among twelve genotypes of pepper (Capsicum). The resulting spectra were converted into a binary matrix consisting of 23 protein data sets, and genetic similarity values were calculated with the FreeTree software and Jaccards coefficient of similarity. We have also been able to identify the presence of certain proteins in the extracts, by checking their masses on online databases.

Keywords: mass spectrometry, time of flight, germplasm, capsicum, genetic variability

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Protein & Peptide Letters

Title: Seed Protein Variation Among Pepper (Capsicum Sp.) Genotypes Revealed By Maldi-Tof Analysis

Volume: 11 Issue: 1

Author(s): Marco Aurelio Caldas de Pinho Pessoa Filho, Carlos Bloch Junior, Danilo Fernandes da Silva Filho, Alexsandro Sobreira Galdino, Rodrigo Maranguape Silva da Cunha, Maria Aparecida Oliveira Alves and Thalles Barbosa Grangeiro

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Keywords: mass spectrometry, time of flight, germplasm, capsicum, genetic variability

Abstract: A method for seed proteome analysis using MALDI-TOF mass spectrometry is described. The data were used to estimate the genetic diversity degree among twelve genotypes of pepper (Capsicum). The resulting spectra were converted into a binary matrix consisting of 23 protein data sets, and genetic similarity values were calculated with the FreeTree software and Jaccards coefficient of similarity. We have also been able to identify the presence of certain proteins in the extracts, by checking their masses on online databases.

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Caldas de Pinho Pessoa Filho Aurelio Marco, Junior Bloch Carlos, Filho Fernandes da Silva Danilo, Galdino Sobreira Alexsandro, Cunha Maranguape Silva da Rodrigo, Alves Aparecida Oliveira Maria and Grangeiro Barbosa Thalles, Seed Protein Variation Among Pepper (Capsicum Sp.) Genotypes Revealed By Maldi-Tof Analysis, Protein & Peptide Letters 2004; 11 (1) . https://dx.doi.org/10.2174/0929866043478482

DOI https://dx.doi.org/10.2174/0929866043478482	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305

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