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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Preliminary X-Ray Crystallographic Analysis of Centrin from Ciliate Euplotes octocarinatus

Author(s): Xiao-Jing He, Liu Li, Hai Hou, Yaqin Zhao, Aihua Liang and Zihe Rao

Volume 12, Issue 6, 2005

Page: [609 - 611] Pages: 3

DOI: 10.2174/0929866054395842

Price: $65

Abstract

Centrins are four-EF-hand Ca2+-binding proteins, which belong to the CaM super family. The centrin from ciliate Euplotes Octocarinatus has been expressed in Escherichia coli, purified and crystallized using the hanging-drop method. Rod-like crystals were grown and diffracted to 2.0 Å. The crystals belong to space group P222 and the unit-cell parameters are a=34.442 Å, b=48.954 Å, c=72.583 Å.

Keywords: centrin, crystallization, preliminary x-ray studies

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