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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Expressed Protein Ligation to Obtain Selectively Modified Aldo/Keto Reductases

Author(s): Michael P.O. Richter and Annette G. Beck-Sickinger

Volume 12, Issue 8, 2005

Page: [777 - 781] Pages: 5

DOI: 10.2174/0929866054864210

Price: $65


Specific enzyme immobilization has moved into the focus for many applications in biochemical research fields. Expressed Protein Ligation (EPL) has been proven to be ideal to selectively label proteins at single positions. Applying this technique to enzymes of the aldo/keto reductase superfamily provides a new approach to generate native or modified redox enzymes for direct and indirect immobilization.

Keywords: expressed protein ligation (epl), site specific covalent immobilization, akr a, artificial redoxproteins, aldo/keto reductase (akr)

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