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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Screening of a Phage Display Library of Exendin-4 Mutants with the Extracellular Domain of Rat GLP-1 Receptor

Author(s): Xiaopu. Yin, Yushu Ma, Meiyun Liu, Weifeng Gao and Dongzhi Wei

Volume 14, Issue 8, 2007

Page: [816 - 821] Pages: 6

DOI: 10.2174/092986607781483679

Price: $65


A phage display library of exendin-4 mutants was screened with the extracellular domain of rat glucagon-like peptide 1 receptor as the target. A novel variant of exendin-4 with higher affinity for the receptor fraction than that of the wild type was identified. The increased affinity was attributed to the substitution of Glu16 by Val16. Although the substitution probably caused an increased entropic cost to the helix region, the linker around Val16 is more flexible resulting in the increase of affinity for the receptor.

Keywords: Exendin-4, GLP-1 receptor, phage display library

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