Expression of a Lipid Transfer Protein in Escherichia coli and Its Phosphorylation by a Membrane-Bound Calcium-Dependent Protein Kinase

Title: Expression of a Lipid Transfer Protein in Escherichia coli and Its Phosphorylation by a Membrane-Bound Calcium-Dependent Protein Kinase

Volume: 14 Issue: 8

Author(s): M.L. Martin, E. Espinosa Vidal and L. de la Canal

Affiliation:

Keywords: Calcium-dependent protein phosphorylation, GST expression system, Helianthus annuus, lipid transfer protein

Abstract: Ha-AP10 is a basic antifungal peptide from sunflower seeds (Helianthus annuus antifungal peptide of 10 kDa) belonging to the family of plant lipid transfer proteins. We report here its expression in E. coli [Glutathione S-transferase (GST) system] and its phosphorylation by endogenous membrane-bound calcium-dependent protein kinases.

Cite this article as:

M.L. Martin , E. Espinosa Vidal and L. de la Canal , Expression of a Lipid Transfer Protein in Escherichia coli and Its Phosphorylation by a Membrane-Bound Calcium-Dependent Protein Kinase, Protein & Peptide Letters 2007; 14 (8) . https://dx.doi.org/10.2174/092986607781483697