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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Overexpression and Purification of the RyR1 Pore-Forming Region

Author(s): Gil Bu Kang, Hye-Eun Song, Dong-Woo Song, Mun-Kyoung Kim, Seong-Hwan Rho, Do Han Kim and Soo Hyun Eom

Volume 14, Issue 8, 2007

Page: [742 - 746] Pages: 5

DOI: 10.2174/092986607781483615

Price: $65


Ryanodine receptor 1 (RyR1) is a large homotetrameric calcium channel that plays a pivotal role in skeletal muscle contraction. Sequence comparison and mutagenesis studies indicate that the pore architecture of RyR1, including the last two transmembrane helices and the luminal loop linking them, is similar to that of the bacterial KcsA K+ channel. Here, we describe the overexpression and purification of the C-terminal polyhistidine-tagged RyR1 pore-forming region. The nonionic detergent lauryldimethylamine oxide (LDAO) was selected for solubilization of the protein based on its ability to extract the protein from the membrane and to maintain it in a monodisperse state. The protein was then purified using nickel-affinity chromatography and gel filtration. Gel filtration analysis confirmed that the RyR1 fragment containing the pore-forming region (amino acids 4829-5037) is sufficient to form a tetramer.

Keywords: Ryanodine receptor 1, pore-forming region, membrane protein expression, purification, system biology

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