Ultrasensitive Staining-Free Protein Detection After PAA Gel Electrophoresis Using Deep UV Fluorescence

Title: Ultrasensitive Staining-Free Protein Detection After PAA Gel Electrophoresis Using Deep UV Fluorescence

Volume: 14 Issue: 7

Author(s): Eugene Riaplov, Qiang Li and Stefan Seeger

Affiliation:

Keywords: Native fluorescence, deep-UV, polyacrylamide, gel electrophoresis, staining free

Abstract: We present the observation of separated protein bands after polyacrylamide (PAA) gel electrophoresis based on the staining-free detection of their ultra violet (UV)-induced fluorescence employing deep UV confocal fluorescence microscopy. Mixtures of the three biological compounds β-Galactosidase (from Escherichia coli), apo-Transferrin (bovine) and bovine serum albumin (BSA) have been separated and a staining free detection limit below 80 pg (7.0x108 molecules) per band has been achieved. This corresponds to ∼270 molecules in the detection volume for confocal microscopy.

Cite this article as:

Eugene Riaplov , Qiang Li and Stefan Seeger , Ultrasensitive Staining-Free Protein Detection After PAA Gel Electrophoresis Using Deep UV Fluorescence, Protein & Peptide Letters 2007; 14 (7) . https://dx.doi.org/10.2174/092986607781483787