Reevaluating the Capability of Taq DNA Polymerase: Long PCR Amplification

Hayan      Lee; Kyung-Nam      Kim; Young      Kee Chae

Abstract

We tested the ability of Taq DNA polymerase (Taq) to amplify long DNA fragments and showed that, if the conditions were set properly, Taq could successfully perform the “long PCR” up to 24 kb. The conditions include: (1) longer primers, (2) a 2-step cycling, and (3) a “long buffer.” We propose that the most important requirements are the survival rate of Taq at high temperatures and that of the primers against the 5 to 3 exonuclease activity of Taq.

Keywords: Taq DNA polymerase, long amplicons, primers, reaction buffer

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Protein & Peptide Letters

Title: Reevaluating the Capability of Taq DNA Polymerase: Long PCR Amplification

Volume: 14 Issue: 4

Author(s): Hayan Lee, Kyung-Nam Kim and Young Kee Chae

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Keywords: Taq DNA polymerase, long amplicons, primers, reaction buffer

Abstract: We tested the ability of Taq DNA polymerase (Taq) to amplify long DNA fragments and showed that, if the conditions were set properly, Taq could successfully perform the “long PCR” up to 24 kb. The conditions include: (1) longer primers, (2) a 2-step cycling, and (3) a “long buffer.” We propose that the most important requirements are the survival rate of Taq at high temperatures and that of the primers against the 5 to 3 exonuclease activity of Taq.

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Lee Hayan, Kim Kyung-Nam and Kee Chae Young, Reevaluating the Capability of Taq DNA Polymerase: Long PCR Amplification, Protein & Peptide Letters 2007; 14 (4) . https://dx.doi.org/10.2174/092986607780363934