Abstract
A simple and sensitive isocratic reverse-phase high performance liquid chromatography method coupled with charged aerosol detection (HPLC-CAD) was developed and validated for the determination of gabapentin concentrations in a pharmaceutical formulation and in biological samples (rat serum and urine). Biological samples were simply treated with acetonitrile and directly injected into the HPLC without any further extraction or derivatization procedures. The separation was achieved on a GraceSmart RP-C18 packed column (250 × 4.6 mm, 5 μm) using a methanol-water (55:45, v/v) mobile phase at a flow rate of 1.1 mL/min at 30 °C. The total run time was less than 8 min. The standard calibration curves were linear (r2 > 0.998) over the range of 2-300 μg/mL for the pharmaceutical formulation, 25-800 μg/mL for rat serum and 50-1600 μg/mL for rat urine. The limits of detection (signal/noise = 3) were 0.5 μg/mL, 10 μg/mL and 15 μg/mL for pharmaceutical formulation, serum and urine, respectively. The intra-day and inter-day precisions in the pharmaceutical formulation analysis ranged between 2.7-3.9% and 3.1-4.4%, respectively. In the rat serum analysis, the intra-day and inter-day precision ranges were 3.5-4.7% and 3.0-9.0%, respectively, while the rat urine analysis intra-day and inter-day precision ranges were 1.9-7.6% and 4.2-9.9%, respectively. The developed HPLC-CAD method successfully determined gabapentin concentrations in commercial gabapentin tablets, as well as in rat serum and urine samples after drug administration.
Keywords: Charged aerosol detector, Commercial drug, Gabapentin, HPLC, Non-derivatization, Serum, Urine, HPLC-CAD, Anti-epileptic drug, Mass spectrometry, Flame ionization detection, Gas chromatography, Capillary electrophoresis
Current Analytical Chemistry
Title: Non-Derivatization Method for the Determination of Gabapentin in Pharmaceutical Formulations, Rat Serum and Rat Urine using High Performance Liquid Chromatography Coupled with Charged Aerosol Detection
Volume: 8 Issue: 1
Author(s): Shaodong Jia, Hee-Seung Lee, Myung-Joo Choi, Sang Hyun Sung, Sang B. Han, Jeong H. Park, Soon-Sun Hong, Sung W. Kwon and Jeongmi Lee
Affiliation:
Keywords: Charged aerosol detector, Commercial drug, Gabapentin, HPLC, Non-derivatization, Serum, Urine, HPLC-CAD, Anti-epileptic drug, Mass spectrometry, Flame ionization detection, Gas chromatography, Capillary electrophoresis
Abstract: A simple and sensitive isocratic reverse-phase high performance liquid chromatography method coupled with charged aerosol detection (HPLC-CAD) was developed and validated for the determination of gabapentin concentrations in a pharmaceutical formulation and in biological samples (rat serum and urine). Biological samples were simply treated with acetonitrile and directly injected into the HPLC without any further extraction or derivatization procedures. The separation was achieved on a GraceSmart RP-C18 packed column (250 × 4.6 mm, 5 μm) using a methanol-water (55:45, v/v) mobile phase at a flow rate of 1.1 mL/min at 30 °C. The total run time was less than 8 min. The standard calibration curves were linear (r2 > 0.998) over the range of 2-300 μg/mL for the pharmaceutical formulation, 25-800 μg/mL for rat serum and 50-1600 μg/mL for rat urine. The limits of detection (signal/noise = 3) were 0.5 μg/mL, 10 μg/mL and 15 μg/mL for pharmaceutical formulation, serum and urine, respectively. The intra-day and inter-day precisions in the pharmaceutical formulation analysis ranged between 2.7-3.9% and 3.1-4.4%, respectively. In the rat serum analysis, the intra-day and inter-day precision ranges were 3.5-4.7% and 3.0-9.0%, respectively, while the rat urine analysis intra-day and inter-day precision ranges were 1.9-7.6% and 4.2-9.9%, respectively. The developed HPLC-CAD method successfully determined gabapentin concentrations in commercial gabapentin tablets, as well as in rat serum and urine samples after drug administration.
Export Options
About this article
Cite this article as:
Jia Shaodong, Lee Hee-Seung, Choi Myung-Joo, Hyun Sung Sang, B. Han Sang, H. Park Jeong, Hong Soon-Sun, W. Kwon Sung and Lee Jeongmi, Non-Derivatization Method for the Determination of Gabapentin in Pharmaceutical Formulations, Rat Serum and Rat Urine using High Performance Liquid Chromatography Coupled with Charged Aerosol Detection, Current Analytical Chemistry 2012; 8 (1) . https://dx.doi.org/10.2174/157341112798472161
DOI https://dx.doi.org/10.2174/157341112798472161 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
Bioanalytical and Biosensor Technologies for Target Molecules
Bioanalytical and biosensor technologies constitute the core of detecting and sensing diverse molecules within living organisms, encompassing proteins, DNA, small molecular metabolites, and beyond. These molecules assume pivotal roles in biological processes, rendering their analysis and sensing indispensable for comprehending biological phenomena and unraveling disease mechanisms. In the current landscape ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
PLGA Nanoparticles and Polysorbate-80-Coated PLGA Nanoparticles Increase the In vitro Antioxidant Activity of Melatonin
Current Drug Delivery Nitric Oxide and Dopamine Neurons. Implications for Parkinsons Disease
Current Medicinal Chemistry - Central Nervous System Agents The Mediterranean and other Dietary Patterns in Secondary Cardiovascular Disease Prevention: A Review
Current Vascular Pharmacology Transition Metal Complexes of Some Azamacrocycles and Their Use in Molecular Recognition
Current Organic Synthesis Choline Transporter CHT Regulation and Function in Cholinergic Neurons
Central Nervous System Agents in Medicinal Chemistry Huntingtin Levels are Elevated in Hippocampal Post-Mortem Samples of Alzheimer’s Disease Brain
Current Alzheimer Research Endothelial Dysfunction and Coronary Artery Spasm
Current Drug Targets - Cardiovascular & Hematological Disorders The Effects of Maternally Administered Methadone, Buprenorphine and Naltrexone on Offspring: Review of Human and Animal Data
Current Neuropharmacology Pharmacodynamics of Memantine: An Update
Current Neuropharmacology Cytoskeletal Integrity as a Drug Target
Current Alzheimer Research Innovative Research Financing for the 21<sup>st</sup> Century: A Case Narrative in How Technology Transfer and Entrepreneurship Supported the Development of a Prototype Cognitive Prosthetic
Technology Transfer and Entrepreneurship (Discontinued) Purinergic Receptors and Pain
Current Pharmaceutical Design Recent Developments in Group I Metabotropic Glutamate Receptor Allosteric Modulators for the Treatment of Psychiatric and Neurological Disorders (2014-May 2015)
Current Topics in Medicinal Chemistry Current Nanotechnological Approaches for an Effective Delivery of Bioactive Drug Molecules to Overcome Drug Resistance Tuberculosis
Current Pharmaceutical Design Other Drugs Acting on Nervous System Associated with QT-Interval Prolongation
Current Drug Safety HR MAS MR Spectroscopy in Metabolic Characterization of Cancer
Current Topics in Medicinal Chemistry Human Internal Jugular Valve M-mode Ultrasound Characterization
Current Neurovascular Research Nanotechnology-Based Platforms for Vaginal Delivery of Peptide Microbicides
Current Medicinal Chemistry Established and Emerging Therapies for Huntingtons Disease
Current Molecular Medicine Disequilibrium and Risk of Falling in the Elderly is a Priority for Health Services
Reviews on Recent Clinical Trials