Abstract
Cannabidiol (CBD) is a cannabinoid found in the Cannabis sativa plant and it is devoid of the typical cognitive and psychological effects of Delta 9-tetrahydrocannabinol (Delta 9-THC). However, studies with animals suggest that CBD presents anxiolytic properties. In the face of evidence of a cannabinoid system in humans, and the growing interest in the therapeutic use of CBD, the purpose of the present study is to develop and validate an analytical methodology for determination of CBD in human plasma. The methodology developed is based on liquid-liquid extraction and gas chromatography/mass spectrometry, and it is linear in the concentration range from 2.5 to 250 ng/mL of plasma, with the limit of detection of 1.0 ng/mL. The precision of inter and intra assays was respectively in the range from 7.5% to 8.2% and from 2.5% to 7.0%. Accuracy of the values of inter and intra assays varied respectively from 1.3% to 8.6% and from 1.2% to 4.4%. Extraction efficiency obtained was in the range of 54.6% to 75.3%. The validated methodology was demonstrated to be rapid, sensitive and suitable for application in clinical studies, as determined by monitoring patients in a controlled administration trial with CBD.
Keywords: Cannabidiol, Human plasma, Gas chromatography/mass spectrometry, Anxiety, Therapeutic drug monitoring, Validation, Chromatographic Analysis, Limit of Detection, Limit of Quantification, Stability, Specificity, Precision, Accuracy
Current Pharmaceutical Analysis
Title: Development and Validation of a Liquid-Liquid Extraction and Gas Chromatography/Mass Spectrometry Methodology for Cannabidiol Analysis in Human Plasma
Volume: 7 Issue: 4
Author(s): Stefania P.S. Camargo, Regina H.C. Queiroz, Wilson R. Malfara, Jose A. de Souza Crippa, Antonio W. Zuardi, Jaime E.C. Hallak and Bruno S. De Martinis
Affiliation:
Keywords: Cannabidiol, Human plasma, Gas chromatography/mass spectrometry, Anxiety, Therapeutic drug monitoring, Validation, Chromatographic Analysis, Limit of Detection, Limit of Quantification, Stability, Specificity, Precision, Accuracy
Abstract: Cannabidiol (CBD) is a cannabinoid found in the Cannabis sativa plant and it is devoid of the typical cognitive and psychological effects of Delta 9-tetrahydrocannabinol (Delta 9-THC). However, studies with animals suggest that CBD presents anxiolytic properties. In the face of evidence of a cannabinoid system in humans, and the growing interest in the therapeutic use of CBD, the purpose of the present study is to develop and validate an analytical methodology for determination of CBD in human plasma. The methodology developed is based on liquid-liquid extraction and gas chromatography/mass spectrometry, and it is linear in the concentration range from 2.5 to 250 ng/mL of plasma, with the limit of detection of 1.0 ng/mL. The precision of inter and intra assays was respectively in the range from 7.5% to 8.2% and from 2.5% to 7.0%. Accuracy of the values of inter and intra assays varied respectively from 1.3% to 8.6% and from 1.2% to 4.4%. Extraction efficiency obtained was in the range of 54.6% to 75.3%. The validated methodology was demonstrated to be rapid, sensitive and suitable for application in clinical studies, as determined by monitoring patients in a controlled administration trial with CBD.
Export Options
About this article
Cite this article as:
P.S. Camargo Stefania, H.C. Queiroz Regina, R. Malfara Wilson, A. de Souza Crippa Jose, W. Zuardi Antonio, E.C. Hallak Jaime and S. De Martinis Bruno, Development and Validation of a Liquid-Liquid Extraction and Gas Chromatography/Mass Spectrometry Methodology for Cannabidiol Analysis in Human Plasma, Current Pharmaceutical Analysis 2011; 7 (4) . https://dx.doi.org/10.2174/157341211797457952
DOI https://dx.doi.org/10.2174/157341211797457952 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Nerve Growth Factor: Structure, Function and Therapeutic Implications for Alzheimers Disease
Current Drug Targets - CNS & Neurological Disorders G-Protein Coupled Receptor Resensitization - Appreciating the Balancing Act of Receptor Function
Current Molecular Pharmacology Activity-Dependent Release of Adenosine: A Critical Re-Evaluation of Mechanism
Current Neuropharmacology Potential of Selected Indian Herbs for COVID-19
Current Traditional Medicine α-Lactalbumin: Of Camels and Cows
Protein & Peptide Letters Tyrosine Hydroxylase Gene: Another Piece of the Genetic Puzzle of Parkinson’s Disease
CNS & Neurological Disorders - Drug Targets Innate Immune System Modulation During Aging: Contributions of Macrophages and Dendritic Cells
Current Immunology Reviews (Discontinued) A Review of Lysergic Acid Diethylamide (LSD) in the Treatment of Addictions: Historical Perspectives and Future Prospects
Current Drug Abuse Reviews Mechanisms of Oxidative Glutamate Toxicity: The Glutamate/Cystine Antiporter System xc¯ as a Neuroprotective Drug Target
CNS & Neurological Disorders - Drug Targets Disease State, Age, Sex, and Post-Mortem Time-Dependent Expression of Proteins in AD vs. Control Frontal Cortex Brain Samples
Current Alzheimer Research Molecular Imaging in the Default Mode Network
Current Molecular Imaging (Discontinued) Meet Our Editorial Board Member:
Current Alzheimer Research Statin-Like Drugs for the Treatment of Brain Cholesterol Loss in Alzheimers Disease
Current Drug Safety Mapping the Corrosion Behavior of AZ91D Magnesium Alloy: Influence of Chloride Concentration and Potentiostatic Polarization
Innovations in Corrosion and Materials Science (Discontinued) Exercise as a platform for pharmacotherapy development in cardiac diseases
Current Pharmaceutical Design Starting Point, Duration of Acquisition and Averaged of Time Series Over Subjects: Issues Related to the Reliability and Accuracy of the Constructed Group Bayesian Network for Resting-state fMRI Studies
Neuroscience and Biomedical Engineering (Discontinued) Ameliorative Effects of Baicalein on an Amyloid-β Induced Alzheimer's Disease Rat Model: A Proteomics Study
Current Alzheimer Research Recent Clinical Evidence for the Treatment of Osteoarthritis: What we have Learned
Reviews on Recent Clinical Trials Biological and Pharmacological Effects of Gamma-oryzanol: An Updated Review of the Molecular Mechanisms
Current Pharmaceutical Design Effects of Prefrontal 40 Hz-Centered EEG Band Neurofeedback on Emotional State and Cognitive Functions in Adolescents
Adolescent Psychiatry