Abstract
Proteomics will contribute greatly to the understanding of gene functions in the post-genomic era. In proteome research, protein digestion is a key procedure prior to mass spectrometry identification. During the past decade, a variety of electromagnetic waves have been employed to accelerate proteolysis. This review focuses on the recent advances and the key strategies of these novel proteolysis approaches for digesting and identifying proteins. The subjects covered include microwave-accelerated protein digestion, infrared-assisted proteolysis, ultraviolet-enhanced protein digestion, laser-assisted proteolysis, and future prospects. It is expected that these novel proteolysis strategies accelerated by various electromagnetic waves will become powerful tools in proteome research and will find wide applications in high throughput protein digestion and identification.
Keywords: Proteomics, Proteolysis, Protease, Electromagnetic waves, Peptide mapping, LC, ESI MS, MALDI-TOF MS, SDS-PAGE, Bovine serum albumin
Current Genomics
Title: Highly Efficient Proteolysis Accelerated by Electromagnetic Waves for Peptide Mapping
Volume: 12 Issue: 6
Author(s): Qiwen Chen, Ting Liu and Gang Chen
Affiliation:
Keywords: Proteomics, Proteolysis, Protease, Electromagnetic waves, Peptide mapping, LC, ESI MS, MALDI-TOF MS, SDS-PAGE, Bovine serum albumin
Abstract: Proteomics will contribute greatly to the understanding of gene functions in the post-genomic era. In proteome research, protein digestion is a key procedure prior to mass spectrometry identification. During the past decade, a variety of electromagnetic waves have been employed to accelerate proteolysis. This review focuses on the recent advances and the key strategies of these novel proteolysis approaches for digesting and identifying proteins. The subjects covered include microwave-accelerated protein digestion, infrared-assisted proteolysis, ultraviolet-enhanced protein digestion, laser-assisted proteolysis, and future prospects. It is expected that these novel proteolysis strategies accelerated by various electromagnetic waves will become powerful tools in proteome research and will find wide applications in high throughput protein digestion and identification.
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Cite this article as:
Chen Qiwen, Liu Ting and Chen Gang, Highly Efficient Proteolysis Accelerated by Electromagnetic Waves for Peptide Mapping, Current Genomics 2011; 12 (6) . https://dx.doi.org/10.2174/138920211797248583
DOI https://dx.doi.org/10.2174/138920211797248583 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
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