Abstract
Fungal laccases are generalists biocatalysts with potential applications that range from bioremediation to novel green processes. Fuelled by molecular oxygen, these enzymes can act on dozens of molecules of different chemical nature, and with the help of redox mediators, their spectrum of oxidizable substrates is further pushed towards xenobiotic compounds (pesticides, industrial dyes, PAHs), biopolymers (lignin, starch, cellulose) and other complex molecules. In recent years, extraordinary efforts have been made to engineer fungal laccases by directed evolution and semi-rational approaches to improve their functional expression or stability. All these studies have taken advantage of Saccharomyces cerevisiae as a heterologous host, not only to secrete the enzyme but also, to emulate the introduction of genetic diversity through in vivo DNA recombination. Here, we discuss all these endeavours to convert fungal laccases into valuable biomolecular platforms on which new functions can be tailored by directed evolution.
Keywords: Directed evolution, functional expression, fungal laccases, high-throughput biomolecular screening, in vivo DNA recombination, Saccharomyces cerevisiae, stability, redox mediators, pesticides, biocatalysts
Current Genomics
Title: Directed Evolution of Fungal Laccases
Volume: 12 Issue: 2
Author(s): Diana Mate, Eva Garcia-Ruiz, Susana Camarero and Miguel Alcalde
Affiliation:
Keywords: Directed evolution, functional expression, fungal laccases, high-throughput biomolecular screening, in vivo DNA recombination, Saccharomyces cerevisiae, stability, redox mediators, pesticides, biocatalysts
Abstract: Fungal laccases are generalists biocatalysts with potential applications that range from bioremediation to novel green processes. Fuelled by molecular oxygen, these enzymes can act on dozens of molecules of different chemical nature, and with the help of redox mediators, their spectrum of oxidizable substrates is further pushed towards xenobiotic compounds (pesticides, industrial dyes, PAHs), biopolymers (lignin, starch, cellulose) and other complex molecules. In recent years, extraordinary efforts have been made to engineer fungal laccases by directed evolution and semi-rational approaches to improve their functional expression or stability. All these studies have taken advantage of Saccharomyces cerevisiae as a heterologous host, not only to secrete the enzyme but also, to emulate the introduction of genetic diversity through in vivo DNA recombination. Here, we discuss all these endeavours to convert fungal laccases into valuable biomolecular platforms on which new functions can be tailored by directed evolution.
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Cite this article as:
Mate Diana, Garcia-Ruiz Eva, Camarero Susana and Alcalde Miguel, Directed Evolution of Fungal Laccases, Current Genomics 2011; 12 (2) . https://dx.doi.org/10.2174/138920211795564322
DOI https://dx.doi.org/10.2174/138920211795564322 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
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