Generic placeholder image

Current Analytical Chemistry


ISSN (Print): 1573-4110
ISSN (Online): 1875-6727

Interactions of Two Ethidium Derivatives with Calf Thymus DNA and Serum Albumins Using Fluorescence Quenching Method

Author(s): N. Akbay, Z. Seferoglu and E. Gokoglu

Volume 6, Issue 4, 2010

Page: [334 - 340] Pages: 7

DOI: 10.2174/1573411011006040334

Price: $65


In the present study, we reported the syntheses and investigation of the modes of binding to DNA and serum albumins of the two ethidium derivatives containing ethyl and methyl chloroformate of both amines at 3-and 8- positions. The interactions of calf thymus DNA (ct-DNA) and serum albumins (BSA and HSA) with the two derivatives, E4 and E5, were investigated using fluorescence quenching method and UV-visible absorption spectra. The obtained data suggest that used derivatives can interact strongly with DNA and serum albumins; there are the formation of derivative-DNA and serum albumin complexes and hydrophobic interactions as the predominant intermolecular forces in stabilizing for each complex. The Stern-Volmer quenching constants, binding constants, binding sites and the corresponding thermodynamic parameters ΔH, ΔS and ΔG were calculated at different temperatures. The results of absorption, ionic strength and iodide ion quenching suggested that the interaction mode of E4 and E5 with DNA was groove binding.

Keywords: Fluorescence Quenching, Stern-Volmer, Calf thymus DNA, Serum albumin.

« Previous

Rights & Permissions Print Export Cite as
© 2023 Bentham Science Publishers | Privacy Policy