Abstract
We have developed a high-pH, pH-shift refolding “Ph-Fold technology”, both for academic research and industrial protein drug development applications. Using this technology, we were able to refold some “difficult-to-refold” proteins, some of which are proven important drug targets and protein drug candidates. The technology is composed of the initial E. coli production of inclusion bodies, the high pH solubilization/pH shift refolding screening technology, which includes automated pH shift refolding screening, and the methods of testing protein refolding without functional assay. This technology, especially the automated refolding system based on the Ph-Fold technology, may help both academic and industrial research in broadening structural genomic research, functional proteomics, and genomic scale drug development efforts.
Keywords: E. coli expression, inclusion bodies, high pH reflolding, pH shift refolding
Current Pharmaceutical Biotechnology
Title: The High pH and pH-Shift Refolding Technology
Volume: 11 Issue: 3
Author(s): Xinli Lin and Tomomi Umetsu
Affiliation:
Keywords: E. coli expression, inclusion bodies, high pH reflolding, pH shift refolding
Abstract: We have developed a high-pH, pH-shift refolding “Ph-Fold technology”, both for academic research and industrial protein drug development applications. Using this technology, we were able to refold some “difficult-to-refold” proteins, some of which are proven important drug targets and protein drug candidates. The technology is composed of the initial E. coli production of inclusion bodies, the high pH solubilization/pH shift refolding screening technology, which includes automated pH shift refolding screening, and the methods of testing protein refolding without functional assay. This technology, especially the automated refolding system based on the Ph-Fold technology, may help both academic and industrial research in broadening structural genomic research, functional proteomics, and genomic scale drug development efforts.
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Cite this article as:
Lin Xinli and Umetsu Tomomi, The High pH and pH-Shift Refolding Technology, Current Pharmaceutical Biotechnology 2010; 11 (3) . https://dx.doi.org/10.2174/138920110791111906
DOI https://dx.doi.org/10.2174/138920110791111906 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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