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Current Pharmaceutical Biotechnology


ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

Development of an Insect Cell-Free System

Author(s): Toru Ezure, Takashi Suzuki, Masamitsu Shikata, Masaaki Ito, Eiji Ando, Toshihiko Utsumi, Osamu Nishimura and Susumu Tsunasawa

Volume 11, Issue 3, 2010

Page: [279 - 284] Pages: 6

DOI: 10.2174/138920110791111997

Price: $65


Cell-free protein synthesis systems offer production of native proteins with high speed, even for the proteins that are toxic to cells. Among cell-free systems, the system derived from insect cells has the potential to carry out posttranslational modifications that are specific to eukaryotic organisms, as occurs in the rabbit reticulocyte system. In this review, we describe development of this insect cell-free system and its applications.

Keywords: Cell-free protein synthesis, 5'-UTR, Freeze-thawing, Insect cell, MALDI-TOF MS, Post-translational modification, Rabbit reticulocyte

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