Development of an Insect Cell-Free System

Title: Development of an Insect Cell-Free System

Volume: 11 Issue: 3

Author(s): Toru Ezure, Takashi Suzuki, Masamitsu Shikata, Masaaki Ito, Eiji Ando, Toshihiko Utsumi, Osamu Nishimura and Susumu Tsunasawa

Affiliation:

Keywords: Cell-free protein synthesis, 5'-UTR, Freeze-thawing, Insect cell, MALDI-TOF MS, Post-translational modification, Rabbit reticulocyte

Abstract: Cell-free protein synthesis systems offer production of native proteins with high speed, even for the proteins that are toxic to cells. Among cell-free systems, the system derived from insect cells has the potential to carry out posttranslational modifications that are specific to eukaryotic organisms, as occurs in the rabbit reticulocyte system. In this review, we describe development of this insect cell-free system and its applications.

Cite this article as:

Ezure Toru, Suzuki Takashi, Shikata Masamitsu, Ito Masaaki, Ando Eiji, Utsumi Toshihiko, Nishimura Osamu and Tsunasawa Susumu, Development of an Insect Cell-Free System, Current Pharmaceutical Biotechnology 2010; 11 (3) . https://dx.doi.org/10.2174/138920110791111997