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Current Pharmaceutical Biotechnology


ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

Silkworm as a Host of Baculovirus Expression

Author(s): Akihiro Usami, Takeo Suzuki, Hidekazu Nagaya, Hiroki Kaki and Seiji Ishiyama

Volume 11 , Issue 3 , 2010

Page: [246 - 250] Pages: 5

DOI: 10.2174/138920110791112013

Price: $65


While the Baculovirus Expression Vector System (BEVS) mainly uses insect cell lines, such as Sf9 cells, the robust high expression system using silkworm has also been developed. We have further improved technologies for enhancement of virus recombination, reduction of proteolytic degradation and aggregation, and more reliable promoters. These developments made it possible to achieve high and soluble expression of recombinant proteins. We review here such technology developments, advantage of using silkworm and some example applications. There are areas where this technology can be further improved as implicated in the end.

Keywords: Baculovirus, silkworm, polyhedrin, cathepsin-like protease, vp39, Dock-tag

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