Abstract
A flow-spectrophotometric method is proposed for the routine determination of tartaric acid in wines. The reaction between tartaric acid and vanadate in acetic media is carried out in flowing conditions and the subsequent colored complex is monitored at 475 nm. The stability of the complex and the corresponding formation constant are presented. The effect of wavelength and pH was evaluated by batch experiments. The selected conditions were transposed to a flowinjection analytical system. Optimization of several flow parameters such as reactor lengths, flow-rate and injection volume was carried out. Using optimized conditions, a linear behavior was observed up to 1000 μg mL-1 tartaric acid, with a molar extinction coefficient of 450 L mg-1 cm-1 and ± 1 % repeatability. Sample throughput was 25 samples per hour. The flow-spectrophotometric method was satisfactorily applied to the quantification of tartaric acid (TA) in wines from different sources. Its accuracy was confirmed by statistical comparison to the conventional Rebelein procedure and to a certified analytical method carried out in a routine laboratory.
Keywords: Spectrophotometry, tartrate, wine, flow injection analysis
Combinatorial Chemistry & High Throughput Screening
Title: Rapid Determination of Tartaric Acid in Wines
Volume: 12 Issue: 7
Author(s): Sandra S.T. Bastos, Paula A.R. Tafulo, Raquel B. Queiros, Cristina D. Matos and M. Goreti F. Sales
Affiliation:
Keywords: Spectrophotometry, tartrate, wine, flow injection analysis
Abstract: A flow-spectrophotometric method is proposed for the routine determination of tartaric acid in wines. The reaction between tartaric acid and vanadate in acetic media is carried out in flowing conditions and the subsequent colored complex is monitored at 475 nm. The stability of the complex and the corresponding formation constant are presented. The effect of wavelength and pH was evaluated by batch experiments. The selected conditions were transposed to a flowinjection analytical system. Optimization of several flow parameters such as reactor lengths, flow-rate and injection volume was carried out. Using optimized conditions, a linear behavior was observed up to 1000 μg mL-1 tartaric acid, with a molar extinction coefficient of 450 L mg-1 cm-1 and ± 1 % repeatability. Sample throughput was 25 samples per hour. The flow-spectrophotometric method was satisfactorily applied to the quantification of tartaric acid (TA) in wines from different sources. Its accuracy was confirmed by statistical comparison to the conventional Rebelein procedure and to a certified analytical method carried out in a routine laboratory.
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Cite this article as:
Bastos S.T. Sandra, Tafulo A.R. Paula, Queiros B. Raquel, Matos D. Cristina and Sales Goreti F. M., Rapid Determination of Tartaric Acid in Wines, Combinatorial Chemistry & High Throughput Screening 2009; 12 (7) . https://dx.doi.org/10.2174/138620709788923728
DOI https://dx.doi.org/10.2174/138620709788923728 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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