Abstract
Previous studies using different techniques have shown that adenoviral-mediated gene transfer to different tissues, including the kidney, is more efficient in neonatal mice. In this study, we report a simple technique that allows an efficient and long term expression of β-galactosidase (β-gal) in the heart of newborn mice. Newborn and adult C57BL6/J mice were subjected to a single retro-orbital venous plexus injection of recombinant adenoviral vectors (rAd) (2 x 10 particles/ g body weight) carrying the lac Z gene. Seven days after the injection, positive β-gal staining was systematically observed in the heart, lung, intestine, liver, kidney and spleen of newborn mice. However, only the heart showed persistent expression of β-gal one year after the initial injection. In contrast, adult mice showed only significant but transient β- gal expression mainly in the liver. In summary, we have found that a single retro-orbital intravenous injection can be used to establish a long-term adenoviral-mediated gene transfer to cardiac cells of newborn mice.
Keywords: Recombinant adenoviral vector, long term gene transfer, newborn mouse, heart, cardiomyocytes
Cardiovascular & Hematological Disorders-Drug Targets
Title: A Simple Technique to Establish a Long-Term Adenovirus Mediated Gene Transfer to the Heart of Newborn Mice
Volume: 9 Issue: 2
Author(s): Marina Jerebtsova, Xuehai Ye and Patricio E. Ray
Affiliation:
Keywords: Recombinant adenoviral vector, long term gene transfer, newborn mouse, heart, cardiomyocytes
Abstract: Previous studies using different techniques have shown that adenoviral-mediated gene transfer to different tissues, including the kidney, is more efficient in neonatal mice. In this study, we report a simple technique that allows an efficient and long term expression of β-galactosidase (β-gal) in the heart of newborn mice. Newborn and adult C57BL6/J mice were subjected to a single retro-orbital venous plexus injection of recombinant adenoviral vectors (rAd) (2 x 10 particles/ g body weight) carrying the lac Z gene. Seven days after the injection, positive β-gal staining was systematically observed in the heart, lung, intestine, liver, kidney and spleen of newborn mice. However, only the heart showed persistent expression of β-gal one year after the initial injection. In contrast, adult mice showed only significant but transient β- gal expression mainly in the liver. In summary, we have found that a single retro-orbital intravenous injection can be used to establish a long-term adenoviral-mediated gene transfer to cardiac cells of newborn mice.
Export Options
About this article
Cite this article as:
Jerebtsova Marina, Ye Xuehai and Ray E. Patricio, A Simple Technique to Establish a Long-Term Adenovirus Mediated Gene Transfer to the Heart of Newborn Mice, Cardiovascular & Hematological Disorders-Drug Targets 2009; 9 (2) . https://dx.doi.org/10.2174/187152909788488645
DOI https://dx.doi.org/10.2174/187152909788488645 |
Print ISSN 1871-529X |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-4063 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Review Metabolomics: A New Approach to Drug Delivery in Perinatology
Current Medicinal Chemistry Developmental Immunotoxicity (DIT) in Drug Safety Testing: Matching DIT Testing to Adverse Outcomes and Childhood Disease Risk
Current Drug Safety Damnacanthal-Induced Anti-Inflammation is Associated with Inhibition of NF-κB Activity
Inflammation & Allergy - Drug Targets (Discontinued) The Anticancer Activity of the N-Terminal CARD-Like Domain of Arginine Deiminase (ADI) from Pseudomonas aeruginosa
Letters in Drug Design & Discovery Current Pharmacologic Management of Pediatric Heart Failure in Congenital Heart Disease
Current Vascular Pharmacology Subject Index To Volume 2
Current Rheumatology Reviews Meet Our Editorial Board Member
Cardiovascular & Hematological Disorders-Drug Targets Crosstalk Between Bioactive Peptide and Intestinal Barrier in Gut Homeostasis
Current Protein & Peptide Science Protein C Inhibitor as an Anti-Disseminated Intravascular Coagulation Agent - Mechanism and Modification
Current Medicinal Chemistry - Cardiovascular & Hematological Agents PET Radiopharmaceuticals for Personalized Medicine
Current Drug Targets Is Erectile Dysfunction an Example of Abnormal Endothelial Function?
Current Vascular Pharmacology Production of Retroviral Vectors: Review
Current Gene Therapy Fullerenes: From Carbon to Nanomedicine
Mini-Reviews in Medicinal Chemistry Drugs and Rhabdomyolysis: From Liver to Kidney
Current Vascular Pharmacology COVID-19 Vaccination in Children: An Open Question
Current Pediatric Reviews Novel Targets for Antiinflammatory and Antiarthritic Agents
Current Pharmaceutical Design Preventing Vascular Graft Failure: Endothelial Cell Seeding and Tissue Engineering
Vascular Disease Prevention (Discontinued) Diabetic Cardiomyopathy and Oxidative Stress: Role of Antioxidants
Cardiovascular & Hematological Agents in Medicinal Chemistry Cardiovascular Considerations of Remdesivir and Favipiravir in the Treatment of COVID-19
Cardiovascular & Hematological Disorders-Drug Targets Identification of Disease-Relevant Genes for Molecularly-Targeted Drug Discovery
Current Cancer Drug Targets