Significance of Ribonucleoside-diphosphate Reductase Subunit M2 in Lung Adenocarcinoma

Title:Significance of Ribonucleoside-diphosphate Reductase Subunit M2 in Lung Adenocarcinoma

Volume: 25 Issue: 2

Author(s): Xiao-Jun Wang, Yun-Xia Huo, Peng-Jun Yang, Jing Gao*Wei-Dong Hu*

Affiliation:

• Department of Respiratory Medicine, Gansu Province People Hospital, Lanzhou, Gansu, PR China
• Department of Medicine, Respiratory Medicine Unit , Karolinska Institute, Stockholm, Sweden
• Department of Pulmonary Medicine, University of Helsinki and Helsinki University Hospital, Helsinki, Finland

• Department of Respiratory Medicine, Gansu Province People Hospital, Lanzhou, Gansu, PR China

Keywords: Lung adenocarcinoma (LUAD), ribonucleoside-diphosphate reductase subunit M2 (RRM2), molecular biomarker, bioinformatic analyses, experimental validation, cancers.

Abstract:

Introduction: The Ribonucleoside-diphosphate Reductase subunit M2 (RRM2) is known to be overexpressed in various cancers, though its specific functional implications remain unclear. This aims to elucidate the role of RRM2 in the progression of Lung Adenocarcinoma (LUAD) by exploring its involvement and potential impact.

Methods: RRM2 data were sourced from multiple databases to assess its diagnostic and prognostic significance in LUAD. We evaluated the association between RRM2 expression and immune cell infiltration, analyzed its function, and explored the effects of modulating RRM2 expression on LUAD cell characteristics through laboratory experiments.

Results: RRM2 was significantly upregulated in LUAD tissues and cells compared to normal counterparts (p < 0.05), with rare genetic alterations noted (approximately 2%). This overexpression clearly distinguished LUAD from normal tissue (area under the curve (AUC): 0.963, 95% confidence intervals (CI): 0.946-0.981). Elevated RRM2 expression was significantly associated with adverse clinicopathological characteristics and poor prognosis in LUAD patients. Furthermore, a positive association was observed between RRM2 expression and immune cell infiltration. Pathway analysis revealed a critical connection between RRM2 and the cell cycle signaling pathway within LUAD. Targeting RRM2 inhibition effectively suppressed LUAD cell proliferation, migration, and invasion while promoting apoptosis. This intervention also modified the expression of several crucial proteins, including the downregulation of CDC25A, CDC25C, RAD1, Bcl-2, and PPM1D and the upregulation of TP53 and Bax (p < 0.05).

Conclusion: Our findings highlight the potential utility of RRM2 expression as a biomarker for diagnosing and predicting prognosis in LUAD, shedding new light on the role of RRM2 in this malignancy.

Cite this article as:

Wang Xiao-Jun, Huo Yun-Xia, Yang Peng-Jun, Gao Jing*, Hu Wei-Dong*, Significance of Ribonucleoside-diphosphate Reductase Subunit M2 in Lung Adenocarcinoma, Current Gene Therapy 2025; 25 (2) . https://dx.doi.org/10.2174/0115665232286359240611051307