Tramadol, a 4-phenyl-piperidine analogue of codeine, is usually marketed as the hydrochloride salt and is a centrally acting analgesic, used for treating moderate to severe pain. In this study, a potentiometric liquid membrane sensors for simple and fast determination of tramadol hydrochloride in pharmaceutical formulation and urine was constructed. For the membrane preparation, tramadol-tetraphenyl borate complexes were employed as electroactive material in the membrane. The wide linear range (10-5-10-1 M), low detection limit (3 μ g/ml), and fast response time (10 s) are the characterizations of the proposed sensors. Validation of the method shows suitability of the sensors for applies in the quality control analysis of tramadol hydrochloride in pharmaceutical formulation and urine. The proposed method is found to be simple, accurate and precise which can be used as a detector for HPLC.
Keywords: Potentiometric sensor, PVC membrane, Tramadol hydrochloride, Calculation chemistry