Background: Schistosomiasis is a neglected tropical parasitic disease caused by trematode worms of the genus schistosoma, which affects approximately 240 million people worldwide. the diagnosis of the disease can be performed by parasitological, molecular, and/or immunological methods, however, the development of new diagnostic methods still essential to guide policy decisions, monitor disease trends and assess the effectiveness of interventions.

Objective: in this sense, the current work summarizes the findings of a systematic review regarding antigens applied in the enzyme-linked immunosorbent assay test, which were patented and published over the last ten years.

Methods: the literature search strategy used medical subject heading (mesh) terms to define as descriptors. “schistosoma mansoni” was used in arrangement with the descriptors “immunoassay”, “enzyme-linked immunosorbent assay”, “elisa”, and “antigens”, using the “and” connector. the patent search was done using keywords, including diagnosis and schistosoma or schistosomiasis or schistosome. several databases were employed for the patent search, such as intellectual property national institute; european patent office; the united states patent and trademark office; patent scope, and google patents.

Results: forty-one articles were retrieved, of which only five met the eligibility criteria. seventeen patents were taken from the databases, and a brief description of the most relevant inventions is given here.

Conclusion: schistosomiasis is considered the most important helminthic disease in worldwide. therefore, it is important to of searching for and develops diagnostic methods based on serology to reduce morbidity and mortality caused by the disease.

Keywords: Schistosoma, schistosomiasis, immunodiagnostic, ELISA, antigens, recombinant proteins.

Gryseels B. Schistosomiasis. Infect Dis Clin North Am 2012; 26: 383-97.
[http://dx.doi.org/10.1016/j.idc.2012.03.004] [PMID: 22632645]
Gryseels B, Polman K, Clerinx J, Kestens L. Human schistosomiasis. Lancet 2006; 368(9541): 1106-18.
[http://dx.doi.org/10.1016/S0140-6736(06)69440-3] [PMID: 16997665]
WHO. Schistosomiasis (Bilharzia).. Available From: https://www.who.int/health-topics/schistosomiasis#tab=tab_1 (Accessed March 17, 2022).
Colley DG, Bustinduy AL, Secor WE, King CH. Human schistosomiasis. Lancet 2014; 383(9936): 2253-64.
[http://dx.doi.org/10.1016/S0140-6736(13)61949-2] [PMID: 24698483]
Hailegebriel T, Nibret E, Munshea A. Prevalence of Schistosoma mansoni and S. haematobium in snail intermediate hosts in africa: a systematic review and meta analysis. J Trop Med 2020; 2020: 1-18.
[http://dx.doi.org/10.1155/2020/8850840] [PMID: 32963554]
Siqueira LP, Fontes DAF, Aguilera CSB, et al. Schistosomiasis: Drugs used and treatment strategies. Acta Trop 2017; 176: 179-87.
[http://dx.doi.org/10.1016/j.actatropica.2017.08.002] [PMID: 28803725]
Giera M, Kaisar MMM, Derks RJE, et al. The Schistosoma mansoni lipidome: Leads for immunomodulation. Anal Chim Acta 2018; 1037: 107-18.
[http://dx.doi.org/10.1016/j.aca.2017.11.058] [PMID: 30292284]
Figliuolo da Paz VR, Figueiredo-Vanzan D, dos Santos Pyrrho A. Interaction and involvement of cellular adhesion molecules in the pathogenesis of Schistosomiasis mansoni. Immunol Lett 2019; 206: 11-8.
[http://dx.doi.org/10.1016/j.imlet.2018.11.011] [PMID: 30503821]
Inobaya MT, Olveda RM, Chau TN, Olveda DU, Ross AG. Prevention and control of schistosomiasis: A current perspective. Res Rep Trop Med 2014; 2014(5): 65-75.
[PMID: 25400499]
Famakinde DO. Molecular context of Schistosoma mansoni transmission in the molluscan environments: A mini-review. Acta Trop 2017; 176: 98-104.
[http://dx.doi.org/10.1016/j.actatropica.2017.07.021] [PMID: 28754250]
da Paz VRF, Sequeira D, Pyrrho A. Infection by Schistosoma mansoni during pregnancy: Effects on offspring immunity. Life Sci 2017; 185: 46-52.
[http://dx.doi.org/10.1016/j.lfs.2017.07.021] [PMID: 28754617]
Coltart C, Whitty CJM. Schistosomiasis in non-endemic countries. Clin Med 2015; 15(1): 67-9.
[http://dx.doi.org/10.7861/clinmedicine.15-1-67] [PMID: 25650202]
Pila EA, Li H, Hambrook JR, Wu X, Hanington PC. Schistosomiasis from a Snail’s Perspective: Advances in snail immunity. Trends Parasitol 2017; 33(11): 845-57.
[http://dx.doi.org/10.1016/j.pt.2017.07.006] [PMID: 28803793]
Chuah C, Gobert GN, Latif B, Heo CC, Leow CY. Schistosomiasis in Malaysia: A review. Acta Trop 2019; 190: 137-43.
[http://dx.doi.org/10.1016/j.actatropica.2018.11.012] [PMID: 30448471]
Masi B, Perles-Barbacaru TA, Bernard M, Viola A. Clinical and preclinical imaging of hepatosplenic schistosomiasis. Trends Parasitol 2020; 36(2): 206-26.
[http://dx.doi.org/10.1016/j.pt.2019.11.007] [PMID: 31864895]
Nigo MM, Odermatt P, Nigo DW, Salieb-Beugelaar GB, Battegay M, Hunziker PR. Morbidity associated with Schistosoma mansoni infection in north-eastern Democratic Republic of the Congo. PLoS Negl Trop Dis 2021; 15(12): e0009375.
[http://dx.doi.org/10.1371/journal.pntd.0009375] [PMID: 34855763]
Panzner U, Excler JL, Kim JH, Marks F, Carter D, Siddiqui AA. Recent advances and methodological considerations on vaccine candidates for human schistosomiasis. Front Trop Dis 2021; 2: 719369.
Kato K, Miura M. Comparative examinations. Jpn J Parasitol 1954; (3): 35.
Katz N, Chaves A, Pellegrino J. A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni. Rev Inst Med Trop São Paulo 1972; 14(6): 397-400.
[PMID: 4675644]
Hoffman WA, Pons JA, Janer JL. The sedimentation concentration method in Schistostomiasis mansoni. PR J Public Health Trop Med 1934; 9(3): 283-91.
Gomes JF, Hoshino-Shimizu S, Dias LCS, Araujo AJSA, Castilho VLP, Neves FAMA. Evaluation of a novel kit (TF Test) for the diagnosis of intestinal parasitic infections. J Clin Lab Anal 2004; 18(2): 132-8.
[http://dx.doi.org/10.1002/jcla.20011] [PMID: 15065214]
Espirito-Santo MCC, Sanchez MCA, Sanchez AR, et al. Evaluation of the sensitivity of IgG and IgM ELISA in detecting Schistosoma mansoni infections in a low endemicity setting. Eur J Clin Microbiol Infect Dis 2014; 33(12): 2275-84.
[http://dx.doi.org/10.1007/s10096-014-2196-6] [PMID: 25030291]
Fernández-Soto P, Gandasegui Arahuetes J, Sánchez Hernández A, López Abán J, Vicente Santiago B, Muro A. A loop-mediated isothermal amplification (LAMP) assay for early detection of Schistosoma mansoni in stool samples: A diagnostic approach in a murine model. PLoS Negl Trop Dis 2014; 8(9): e3126.
[http://dx.doi.org/10.1371/journal.pntd.0003126] [PMID: 25187956]
Fuss A, Mazigo HD, Tappe D, Kasang C, Mueller A. Comparison of sensitivity and specificity of three diagnostic tests to detect Schistosoma mansoni infections in school children in Mwanza region, Tanzania. PLoS One 2018; 13(8): e0202499.
[http://dx.doi.org/10.1371/journal.pone.0202499] [PMID: 30133490]
Wami WM, Nausch N, Bauer K, et al. Comparing parasitological vs serological determination of Schistosoma haematobium infection prevalence in preschool and primary school-aged children: Implications for control programmes. Parasitology 2014; 141(14): 1962-70.
[http://dx.doi.org/10.1017/S0031182014000213] [PMID: 24679476]
Hinz R, Schwarz NG, Hahn A, Frickmann H. Serological approaches for the diagnosis of schistosomiasis - A review. Mol Cell Probes 2017; 31: 2-21.
[http://dx.doi.org/10.1016/j.mcp.2016.12.003] [PMID: 27986555]
Pontes LA, Dias-Neto E, Rabello A. Detection by polymerase chain reaction of Schistosoma mansoni DNA in human serum and feces. Am J Trop Med Hyg 2002; 66(2): 157-62.
[http://dx.doi.org/10.4269/ajtmh.2002.66.157] [PMID: 12135287]
Mackay IM. Real-time PCR in the microbiology laboratory. Clin Microbiol Infect 2004; 10(3): 190-212.
[http://dx.doi.org/10.1111/j.1198-743X.2004.00722.x] [PMID: 15008940]
Gomes LI, Enk MJ, Rabello A. Diagnosing schistosomiasis: Where are we? Rev Soc Bras Med Trop 2014; 47(1): 3-11.
[http://dx.doi.org/10.1590/0037-8682-0231-2013] [PMID: 24553804]
Hussein HM, El-Tonsy MM, Tawfik RA, Ahmed SAEG. Experimental study for early diagnosis of prepatent schistosomiasis mansoni by detection of free circulating DNA in serum. Parasitol Res 2012; 111(1): 475-8.
[http://dx.doi.org/10.1007/s00436-012-2822-0] [PMID: 22290447]
Weerakoon K, Gordon C, McManus D. DNA diagnostics for schistosomiasis control. Trop Med Infect Dis 2018; 3(3): 81.
[http://dx.doi.org/10.3390/tropicalmed3030081] [PMID: 30274477]
Weerakoon KGAD, Gobert GN, Cai P, McManus DP. Advances in the diagnosis of human schistosomiasis. Clin Microbiol Rev 2015; 28(4): 939-67.
[http://dx.doi.org/10.1128/CMR.00137-14] [PMID: 26224883]
Carvalho GBF, Resende DM, Siqueira LMV, et al. Selecting targets for the diagnosis of Schistosoma mansoni infection: An integrative approach using multi-omic and immunoinformatics data. PLoS One 2017; 12(8): e0182299.
[http://dx.doi.org/10.1371/journal.pone.0182299] [PMID: 28817585]
Fernández-Soto P, Avendaño C, Sala-Vizcaíno A, et al. Molecular markers for detecting Schistosoma species by loop-mediated isothermal amplification. Dis Markers 2020; 2020: 1-11.
[http://dx.doi.org/10.1155/2020/8042705] [PMID: 32774514]
Graeff-Teixeira C, Favero V, de Souza RP, et al. Use of Schistosoma mansoni Soluble Egg Antigen (SEA) for antibody detection and diagnosis of schistosomiasis: The need for improved accuracy evaluations of diagnostic tools. Acta Trop 2021; 215: 105800.
[http://dx.doi.org/10.1016/j.actatropica.2020.105800] [PMID: 33352167]
de Souza C, Lopes MD, De Oliveira FM, et al. Rational selection of immunodominant and preserved epitope Sm043300e from Schistosoma mansoni and design of a chimeric molecule for biotechnological purposes. Mol Immunol 2018; 93: 133-43.
[http://dx.doi.org/10.1016/j.molimm.2017.11.019] [PMID: 29175593]
Carneiro TR, Pinheiro MCC, Oliveira SM, Hanemann ALP, Queiroz JAN, Bezerra FSM. Increased detection of schistosomiasis with Kato-Katz and SWAP-IgG-ELISA in a Northeastern Brazil low-intensity transmission area. Rev Soc Bras Med Trop 2012; 45(4): 510-3.
[http://dx.doi.org/10.1590/S0037-86822012000400019] [PMID: 22930048]
Sarhan RM, Aminou HA, Saad GAR, Ahmed OA. Comparative analysis of the diagnostic performance of adult, cercarial and egg antigens assessed by ELISA, in the diagnosis of chronic human Schistosoma mansoni infection. Parasitol Res 2014; 113(9): 3467-76.
[http://dx.doi.org/10.1007/s00436-014-4017-3] [PMID: 25028207]
Wilkins PP, Keystone JS. Schistosomiasis serology is valuable and reliable. Clin Infect Dis 2013; 56(2): 312.
[http://dx.doi.org/10.1093/cid/cis827] [PMID: 22997211]
Grenfell RFQ, Coelho PMZ, Taboada D, de Mattos ACA, Davis R, Harn DA. Newly established monoclonal antibody diagnostic assays for Schistosoma mansoni direct detection in areas of low endemicity. PLoS One 2014; 9(1): e87777.
[http://dx.doi.org/10.1371/journal.pone.0087777] [PMID: 24498191]
DuVall AS, Muchiri EM, Malhotra I, et al. Development of a specimen-sparing multichannel bead assay to detect antiparasite IgG4 for the diagnosis of Schistosoma and Wuchereria infections on the coast of Kenya. Am J Trop Med Hyg 2014; 90(4): 638-45.
[http://dx.doi.org/10.4269/ajtmh.13-0292] [PMID: 24515945]
Silveira AMS, Costa EGD, Ray D, et al. Evaluation of the CCA Immuno-Chromatographic test to diagnose Schistosoma mansoni in minas gerais state, Brazil. PLoS Negl Trop Dis 2016; 10(1): e0004357.
[http://dx.doi.org/10.1371/journal.pntd.0004357] [PMID: 26752073]
Smith H, Doenhoff M, Aitken C, et al. Comparison of Schistosoma mansoni soluble cercarial antigens and soluble egg antigens for serodiagnosing schistosome infections. PLoS Negl Trop Dis 2012; 6(9): e1815.
[http://dx.doi.org/10.1371/journal.pntd.0001815] [PMID: 23029577]
Wilson MS, Mentink-Kane MM, Pesce JT, Ramalingam TR, Thompson R, Wynn TA. Immunopathology of schistosomiasis. Immunol Cell Biol 2007; 85(2): 148-54.
[http://dx.doi.org/10.1038/sj.icb.7100014] [PMID: 17160074]
Ogongo P, Nyakundi RK, Chege GK, Ochola L. The road to elimination: Current state of schistosomiasis research and progress towards the end game. Front Immunol 2022; 13: 846108.
[http://dx.doi.org/10.3389/fimmu.2022.846108] [PMID: 35592327]
Grenfell RFQ, Martins W, Enk M, et al. Schistosoma mansoni in a low-prevalence area in Brazil: The importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays. Mem Inst Oswaldo Cruz 2013; 108(3): 328-34.
[http://dx.doi.org/10.1590/S0074-02762013000300011] [PMID: 23778663]
Oyeyemi OT, Corsini CA, Gonçalves G, de Castro Borges W, Grenfell RFQ. Evaluation of Schistosomula Crude Antigen (SCA) as a diagnostic tool for Schistosoma mansoni in low endemic human population. Sci Rep 2021; 11(1): 10530.
[http://dx.doi.org/10.1038/s41598-021-89929-3] [PMID: 34006964]
de Oliveira Lopes D, de Oliveira FM, do Vale Coelho IE, et al. Identification of a vaccine against schistosomiasis using bioinformatics and molecular modeling tools. Infect Genet Evol 2013; 20: 83-95.
[http://dx.doi.org/10.1016/j.meegid.2013.08.007] [PMID: 23973434]
Tanaka M, Kildemoes AO, Chadeka EA, et al. Potential of antibody test using Schistosoma mansoni recombinant serpin and RP26 to detect light-intensity infections in endemic areas. Parasitol Int 2021; 83: 102346.
[http://dx.doi.org/10.1016/j.parint.2021.102346] [PMID: 33857597]
Chen JH, Zhang T, Ju C, et al. An integrated immunoproteomics and bioinformatics approach for the analysis of Schistosoma japonicum tegument proteins. J Proteomics 2014; 98: 289-99.
[http://dx.doi.org/10.1016/j.jprot.2014.01.010] [PMID: 24448400]
Silva-Moraes V, Shollenberger LM, Castro-Borges W, Rabello ALT, Harn DA, Medeiros LCS, et al. Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil. PLoS Negl Trop Dis 2019; 13(3): e0006974.
Martins V de P, Oliveira SC, de Morais SB. Recombinant protein, method and kit for diagnosis of schistosomiasis, vaccine composition and use. Patent BR102015027298A2, 2015.
Yibo C, Junhu C, Wei H, Bin X. Japanese schistosomiasis SjPPase recombinant antigen proteins and its application in efficacy assessment. Patent CN107217045A, 2017.
Xiaoting W, Yang D, Jianrong D, Yuntian X, Guoli Q, Jianxia T, et al. schistosoma Japonicum Heat Shock Protein (SjHSP90) recombinant protein and application thereof to schistosomiasis diagnosis and treatment effect evaluation. Patent CN103724414A, 2014.
Chuan S, Xiaojun C, Sha Z. Application of SHSP60 for preparing diagnostic reagent for monitoring comprehensive hepatic pathology injury degree of schistosomiasis patient. Patent CN103063846B, 2012.
Wei H, Yan L, Bin X, Wei M, Chuan Y, Yibo C, et al. Schistosoma Japonicum Calcium-Binding Ef-Hand Domain Containing Protein (SjEFCAB) recombinant antigen protein and preparation method and application thereof. Patent CN103374567A, 2012.
Liu S, Chen Q. Schistosoma japonicum katsurada recombinant protein SjSAPLP4 as well as encoding gene and application thereof. Patent CN105384803A, 2015.
Liu S, Chen Q. Schistosoma japonicum katsurada recombinant protein SjSAPLP5 as well as encoding gene and application thereof. Patent CN105254732A,, 2015.
Yan L, Wei H, Bin X, Chuan Q, Wei M, Yibo C, et al. Schistosoma japonicum SjSap recombinant antigen protein and preparation method and use. Patent CN103102404B, 2012.
Tendler M, Ramos CRR, Simpson AJG. Synthetic gene for expression of sm-14 in Pichia pastoris, processes of production and purification of sm-14 and its use for vaccination and diagnosis. Patent WO2012034197A1, 2013.
Feng Liu. Schistosoma japonicum cathepsin B gene, protein coded by schistosoma japonicum cathepsin B gene and their use. CN104212781A, 2013.
Xiaorong S, Xiaorong L, Jilong S, Qingli L, Yuansheng H, Zhengrong Z, et al. Schistosomiasis detection reagent based on serum photeomics screening and preparation method thereof. Patent CN101799468B, 2012.
Chuanxin Y, Lijun S, Wei Z, Yousheng L, Xuren Y, Wei W, et al. Schistosoma japonicum 23kDa membrane protein big hydrophilic peptide segment fusion protein and application thereof in schistosome infection immune diagnosis. Patent CN102167748A, 2012.
Li M, Xu X, Wu Y. Screen of Schistosoma mansoni diagnostic antigen and use. Patent WO2015172706A1,, 2014.
Liu S, Chen Q, Hou N, Pu X. Recombinant fusion protein ShSAP of Egyptian schistosome and application thereof in schistosomiasis immune diagnosis. Patent CN110564734A, 2019.
Liu S, Chen Q, Hou N, Pu X. Schistosoma mansoni recombinant fusion protein SmSAP and application thereof in schistosomiasis immune diagnosis. Patent CN110627910A, 2019.
Galdino AS, Campos Da Paz MLG, Machado JM, Gonçalves AAM, Ramos LDS, Nogueira LM, et al. Immunogenic chimera with biotechnological applications for the diagnosis of Schistosoma mansoni. Patent BR102018007867A2, 2018.
Galdino AS, Dias DS, Nogueira LM, Ribeiro AF, Oliveira FM, Lopes DDO, et al. Recombinant multiepitope protein and its process of obtaining it for use in the diagnosis of schistosomiasis. Patent BR102016005202A2, 2016.
Pérez-Morales D, Espinoza B. The role of small heat shock proteins in parasites. Cell Stress Chaperones 2015; 20(5): 767-80.
[http://dx.doi.org/10.1007/s12192-015-0607-y] [PMID: 26045203]
Moser D, Doenhoff MJ, Klinkert MQ. A stage-specific calcium-binding protein expressed in eggs of Schistosoma mansoni. Mol Biochem Parasitol 1992; 51(2): 229-38.
[http://dx.doi.org/10.1016/0166-6851(92)90073-S] [PMID: 1574081]
Chazin WJ. Relating form and function of EF-hand calcium binding proteins. Acc Chem Res 2011; 44(3): 171-9.
[http://dx.doi.org/10.1021/ar100110d] [PMID: 21314091]
Lewit-Bentley A, Réty S. EF-hand calcium-binding proteins. Curr Opin Struct Biol 2000; 10(6): 637-43.
[http://dx.doi.org/10.1016/S0959-440X(00)00142-1] [PMID: 11114499]
Herzberg O, James MNG. Structure of the calcium regulatory muscle protein troponin-C at 2.8 Å resolution. Nature 1985; 313(6004): 653-9.
[http://dx.doi.org/10.1038/313653a0] [PMID: 3974698]
Boguta G, Stepkowski D, Bierzyński A. Theoretical estimation of the calcium-binding constants for proteins from the troponin C superfamily based on a secondary structure prediction method I. Estimation procedure. J Theor Biol 1988; 135(1): 41-61.
[http://dx.doi.org/10.1016/S0022-5193(88)80173-5] [PMID: 3256716]
Maeda N, Zhu DX, Fitch WM. Amino acid sequences of lower vertebrate parvalbumins and their evolution: Parvalbumins of boa, turtle, and salamander. Mol Biol Evol 1984; 1(6): 473-88.
[PMID: 6599978]
Derancourt J, Haiech J, Pechère JF. Binding of calcium by parvalbumin fragments. Biochim Biophys Acta Protein Struct 1978; 532(2): 373-5.
[http://dx.doi.org/10.1016/0005-2795(78)90592-5] [PMID: 623786]
Cheung WY. Calmodulin plays a pivotal role in cellular regulation. Science 1979; 207(4426): 19-27.
Pallen CJ, Sharma RK, Wang JH. Modulation of cAMP effects by Ca2+/calmodulin. BioEssays 1985; 2(3): 113-7.
Silva-Moraes V, Ferreira JMS, Coelho PMZ, Grenfell RFQ. Biomarkers for schistosomiasis: Towards an integrative view of the search for an effective diagnosis. Acta Trop 2014; 132: 75-9.
[http://dx.doi.org/10.1016/j.actatropica.2013.12.024] [PMID: 24412728]
Liu S, Zhou X, Piao X, et al. Saposin-like proteins, a multigene family of Schistosoma Species, are biomarkers for the immunodiagnosis of schistosomiasis japonica. J Infect Dis 2016; 214(8): 1225-34.
[http://dx.doi.org/10.1093/infdis/jiw188] [PMID: 27190177]
Skelly PJ, Da’dara AA, Li XH, Castro-Borges W, Wilson RA. Schistosome feeding and regurgitation. PLoS Pathog 2014; 10(8): e1004246.
[http://dx.doi.org/10.1371/journal.ppat.1004246] [PMID: 25121497]
Coulibaly JT, N’Gbesso YK, Knopp S, et al. Accuracy of urine circulating cathodic antigen test for the diagnosis of Schistosoma mansoni in preschool-aged children before and after treatment. PLoS Negl Trop Dis 2013; 7(3): e2109.
[http://dx.doi.org/10.1371/journal.pntd.0002109] [PMID: 23556011]
Koukounari A, Donnelly CA, Moustaki I, et al. A latent Markov modelling approach to the evaluation of circulating cathodic antigen strips for schistosomiasis diagnosis pre- and post praziquantel treatment in Uganda. PLOS Comput Biol 2013; 9(12): e1003402.
[http://dx.doi.org/10.1371/journal.pcbi.1003402] [PMID: 24367250]
Ochodo EA, Gopalakrishna G, Spek B, et al. Circulating antigen tests and urine reagent strips for diagnosis of active schistosomiasis in endemic areas. Cochrane Libr 2015; 2015(3): CD009579.
[http://dx.doi.org/10.1002/14651858.CD009579.pub2] [PMID: 25758180]
Karbalaei M, Rezaee SA, Farsiani H. Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins. J Cell Physiol 2020; 235(9): 5867-81.
[http://dx.doi.org/10.1002/jcp.29583] [PMID: 32057111]

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