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Current Bioactive Compounds

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ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

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Research Article

Study of the Protective Properties of 2-Ethyl-6-Methyl-3-Hydroxypyridine Malate in the Model of In vitro-Induced Oxidative Stress in Myoblast Cell Culture

Author(s): Vladimir A. Furalyov, Vladimir G. Kukes, Albina А. Gazdanova, Olga K. Parfenova, Nikita G. Sidorov and Roman V. Kurkin*

Volume 19, Issue 6, 2023

Published on: 18 January, 2023

Article ID: e221122211123 Pages: 6

DOI: 10.2174/1573407219666221122122346

open access plus

Abstract

Background: A cellular model of oxidative stress induced by hydrogen peroxide in the primary culture of myoblasts was obtained by in vitro experiments, and the possibility of exogenous regulation of the cytotoxic effect using 2-ethyl-6-methyl-3-hydroxypyridine malate (ethoxidol) was studied. Moreover, the influence of oxidative stress and the effect of ethoxidol on the intracellular expression of such an important biomarker as myostatin was investigated.

Methods: Hydrogen peroxide was used to induce oxidative stress. The effect of hydrogen peroxide on the rate of myoblast proliferation was studied by measuring the reduction level of (3-(4,5- dimethylthiazole-2-yl))-2,5-diphenyltetrazolium bromide. To measure the expression of myostatin, a real-time polymerase chain reaction (PCR-RV) method was used.

Results: During the work, it was clearly demonstrated that hydrogen peroxide has a significant cytostatic effect on myoblasts in vitro, inhibiting their proliferation. Ethoxidol in physiological concentration did not show toxic effects and did not inhibit cell proliferation. This antioxidant revealed a statistically significant protective effect on the cytostatic effect of hydrogen peroxide on myoblasts. In addition, this compound inhibited the expression of myostatin mRNA caused by exposure to hydrogen peroxide as a negative regulator of growth and differentiation of muscle tissue that occurs in response to exposure to reactive oxygen species.

Conclusion: Hydrogen peroxide is one of the highly active forms of oxygen and has a significant cytostatic effect on myoblasts in vitro, suppressing their proliferation. 2-ethyl-6-methyl-3- hydroxypyridine malate neutralizes the toxic effect of peroxide, thereby indirectly having a positive effect on the rate of myoblast proliferation in vitro.

Keywords: Oxidative stress, hydrogen peroxide, ethoxidol, protective effect, myoblasts, myostatin, mRNA.

Graphical Abstract

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