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Nanoscience & Nanotechnology-Asia

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ISSN (Print): 2210-6812
ISSN (Online): 2210-6820

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Research Article

Optimization and Fabrication of Curcumin Loaded Solid Lipid Nanoparticles Using Box-Behnken Design for Nasal Delivery

Author(s): Sherry Sharma, Jai Bharti Sharma, Shailendra Bhatt* and Manish Kumar

Volume 12, Issue 6, 2022

Published on: 24 November, 2022

Article ID: e031122210625 Pages: 12

DOI: 10.2174/2210681213666221103151333

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Abstract

Aims: To prepare curcumin loaded solid lipid nanoparticles for nasal administration using Box-Behnken design.

Background: The effectiveness of curcumin in neurological disorders is widely studied by various researchers, but its use is limited due to its poor bioavailability. The brain-targeting efficiency of curcumin can be improved using solid lipid nanoparticles via nasal administration.

Objective: In the present work, Curcumin loaded solid lipid nanoparticles (CUR-SLN) were formulated and optimized for nasal administration.

Methods: Based on solubility studies, cetostearyl alcohol and poloxamer 407 were selected as lipid and surfactant, respectively. Box-Behnken design (BBD) was used to analyze the effects of drug-to-lipid ratio (X1), surfactant concentration (X2) and homogenization time (X3) on particle size (Y1) and % entrapment efficiency (Y2). The CUR-SLN were formulated by the high shear homogenization method. The optimized formulation was evaluated for DSC, TEM, drug release and ex-vivo studies.

Results: Good results were obtained for the particle size and entrapment efficiency analyzed using BBD. The optimized formulation of CUR-SLN obtained using BBD was observed with a particle size of 96.09 nm and % EE of 78.23. In-vitro release of the drug was found to be 82.93± 0.15% after 8 hours. DSC studies revealed that crystalline form of curcumin changed to an amorphous form in SLN. TEM results of optimized CUR-SLN were in correlation with the results obtained using a zeta sizer. No harmful effects were observed on nasal mucosa in the histopathology study.

Conclusion: The SLN can be safely utilized for the intranasal administration of curcumin.

Keywords: Intranasal route, curcumin, solid lipid nanoparticle, cetostearyl alcohol, poloxamer407, box-behnken design.

Graphical Abstract

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