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Pharmaceutical Nanotechnology

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ISSN (Print): 2211-7385
ISSN (Online): 2211-7393

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Research Article

An Exploration of Herbal Extracts Loaded Phyto-phospholipid Complexes (Phytosomes) Against Polycystic Ovarian Syndrome: Formulation Considerations

Author(s): Ruchi Tiwari, Gaurav Tiwari*, Shubham Sharma and Vadivelan Ramachandran

Volume 11, Issue 1, 2023

Published on: 13 December, 2022

Page: [44 - 55] Pages: 12

DOI: 10.2174/2211738510666220919125434

Price: $65

Abstract

Background: Herbal preparations with low oral bioavailability have a fast first-pass metabolism in the gut and liver. To offset these effects, a method to improve absorption and, as a result, bioavailability must be devised.

Objective: The goal of this study was to design, develop, and assess the in vivo toxicity of polyherbal phytosomes for ovarian cyst therapy.

Methods: Using antisolvent and rotational evaporation procedures, phytosomes containing phosphatidylcholine and a combination of herbal extracts (Saraca asoca, Bauhinia variegata, and Commiphora mukul) were synthesized. For a blend of Saraca asoca, Bauhinia variegata, and Commiphora mukul, Fourier-transform infrared spectroscopy (FTIR), preformulation investigations, qualitative phytochemical screening, and UV spectrophotometric tests were conducted. Scanning electron microscopy (SEM), zeta potential, ex vivo release, and in vivo toxicological investigations were used to examine phytosomes.

Results: FTIR studies suggested no changes in descriptive peaks in raw and extracted herbs, although the intensity of peaks was slightly reduced. Zeta potential values between -20.4 mV to - 29.6 mV suggested stable phytosomes with an accepted particle size range. Percentage yield and entrapment efficiency were directly correlated to the amount of phospholipid used. Ex vivo studies suggested that the phytosomes with low content of phospholipids showed good permeation profiles. There was no difference in clinical indications between the extract-loaded phytosomes group and the free extract group in in vivo toxicological or histopathological examinations.

Conclusion: The findings of current research work suggested that the optimized phytosomes based drug delivery containing herbal extracts as bioenhancers has the potential to improve the bioavailability of hydrophobic extracts.

Keywords: Extracts of Saraca asoca, Bauhinia variegata, Commiphora mukul, qualitative phytochemical screening, phytophospholipid complexes (phytosomes), ex vivo, in vivo toxicological studies.

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[1]
Chandrasekar V, Singh AV, Maharjan RS, et al. Perspectives on the technological aspects and biomedical applications of virus‐like particles/nanoparticles in reproductive biology: Insights on the medicinal and toxicological outlook. Adv NanoBiomed Res 2022; 2(8): 2200010.
[http://dx.doi.org/10.1002/anbr.202200010]
[2]
Arentz S, Abbott JA, Smith CA, Bensoussan A. Herbal medicine for the management of polycystic ovary syndrome (PCOS) and associated oligo/amenorrhoea and hyperandrogenism; a review of the laboratory evidence for effects with corroborative clinical findings. BMC Complement Altern Med 2014; 14(1): 511-30.
[http://dx.doi.org/10.1186/1472-6882-14-511] [PMID: 25524718]
[3]
Kwon CY, Cho IH, Park KS. Therapeutic effects and mechanisms of herbal medicines for treating polycystic ovary syndrome: A review. Front Pharmacol 2020; 11: 1192.
[http://dx.doi.org/10.3389/fphar.2020.01192] [PMID: 32903374]
[4]
Pandey TA, Pandey I, Zamboni P, et al. Traditional herbal remedies with a multifunctional therapeutic approach as an implication in COVID-19 associated co-infections. Coatings 2020; 10(8): 761.
[http://dx.doi.org/10.3390/coatings10080761]
[5]
Abasian Z, Rostamzadeh A, Mohammadi M, Hosseini M, Rafieian-kopaei M. A review on role of medicinal plants in polycystic ovarian syndrome: Pathophysiology, neuroendocrine signaling, therapeutic status and future prospects. Middle East Fertil Soc J 2018; 23(4): 255-62.
[http://dx.doi.org/10.1016/j.mefs.2018.04.005]
[6]
Yang H, Kim HJ, Pyun BJ, Lee HW. Licorice ethanol extract improves symptoms of polycytic ovary syndrome in Letrozole-induced female rats. Integr Med Res 2018; 7(3): 264-70.
[http://dx.doi.org/10.1016/j.imr.2018.05.003] [PMID: 30271715]
[7]
Shelar A, Sangshetti J, Chakraborti S, Singh AV, Patil R, Gosavi S. Helminthicidal and larvicidal potentials of biogenic silver nanoparticles synthesized from medicinal plant Momordica charantia. Med Chem 2019; 15(7): 781-9.
[http://dx.doi.org/10.2174/1573406415666190430142637] [PMID: 31208313]
[8]
Shelar A, Singh AV, Dietrich P, et al. Emerging cold plasma treatment and machine learning prospects for seed priming: A step towards sustainable food production. RSC Advances 2022; 12(17): 10467-88.
[http://dx.doi.org/10.1039/D2RA00809B] [PMID: 35425017]
[9]
Saiyed A, Jahan N, Makbul SAA, Ansari M, Bano H, Habib SH. Effect of combination of Withania somnifera Dunal and Tribulus terrestris Linn on letrozole induced polycystic ovarian syndrome in rats. Integr Med Res 2016; 5(4): 293-300.
[http://dx.doi.org/10.1016/j.imr.2016.10.002] [PMID: 28462131]
[10]
Hosseinkhani A, Asadi N, Pasalar M, Zarshenas MM. Traditional Persian medicine and management of metabolic dysfunction in polycystic ovary syndrome. J Tradit Complement Med 2018; 8(1): 17-23.
[http://dx.doi.org/10.1016/j.jtcme.2017.04.006] [PMID: 29321985]
[11]
Amresh G, Agarwal VK, Rao CV. Self microemulsifying formulation of Lagerstroemia speciosa against chemically induced hepatotoxicity. J Tradit Complement Med 2018; 8(1): 164-9.
[http://dx.doi.org/10.1016/j.jtcme.2017.05.005] [PMID: 29322005]
[12]
Behuria HG, Biswal BK, Sahu SK. Electroformation of liposomes and phytosomes using copper electrode. J Liposome Res 2021; 31(3): 255-66.
[http://dx.doi.org/10.1080/08982104.2020.1800729] [PMID: 32703044]
[13]
Minari JB, Okelola CA, Ugochukwu NC. Analysis of Kras gene from induced pancreatic cancer rats administered with Momordica charantia and Ocimum ba s ilicum leaf extracts. J Tradit Complement Med 2018; 8(2): 282-8.
[http://dx.doi.org/10.1016/j.jtcme.2017.04.003] [PMID: 29736383]
[14]
Liao WT, Chiang JH, Li CJ, Lee MT, Su CC, Yen HR. Investigation on the use of traditional chinese medicine for polycystic ovary syndrome in a nationwide prescription database in Taiwan. J Clin Med 2018; 7(7): 179.
[http://dx.doi.org/10.3390/jcm7070179] [PMID: 30037150]
[15]
Gu Y-E, Wang F-F, Yang D-X, et al. Chinese herbal medicine alleviating hyperandrogenism of PCOS rats through regulating PPARG1 and HDAC3 expression in the ovaries. Afr J Tradit Complement Altern Med 2015; 12(2): 6-11.
[http://dx.doi.org/10.4314/ajtcam.v12i2.2]
[16]
Pachiappan S, Ramalingam K, Balasubramanian A. A review on phytomedicine and their mechanism of action on PCOS. Int J Curr Res Rev 2020; 12(23): 81-90.
[http://dx.doi.org/10.31782/IJCRR.2020.122322]
[17]
Mehta MN, Jayaraman M, Agarwal S. Holistic approach in the management of PCOS: A pilot study of an online PCOS-reversal program. Int J Reprod Contracept Obstet Gynecol 2018; 7(7): 2829.
[http://dx.doi.org/10.18203/2320-1770.ijrcog20182890]
[18]
Lu M, Qiu Q, Luo X, et al. Phyto-phospholipid complexes (phytosomes): A novel strategy to improve the bioavailability of active constituents. Asian J Pharm Sci 2019; 14(3): 265-74.
[http://dx.doi.org/10.1016/j.ajps.2018.05.011] [PMID: 32104457]
[19]
Sravanthi M, Pradesh A. Phytosomes: A novel drug delivery for herbal extracts. Int J Pharm Sci Res 2013; 4(3): 949-59.
[20]
Alharbi WS, Almughem FA, Almehmady AM, et al. Phytosomes as an emerging nanotechnology platform for the topical delivery of bioactive phytochemicals. Pharmaceutics 2021; 13(9): 1475.
[http://dx.doi.org/10.3390/pharmaceutics13091475] [PMID: 34575551]
[21]
Archer MA, Kumadoh D, Yeboah GN, et al. Formulation and evaluation of capsules containing extracts of Cassia sieberiana for improved therapeutic outcome. Sci Am 2020; 10: e00609.
[http://dx.doi.org/10.1016/j.sciaf.2020.e00609]
[22]
Pharmacopoeia B. 2013. Version 17.0 Available from: https://www.pharmacopoeia.com/
[23]
Islam N, Irfan M, Hussain T, et al. Piperine phytosomes for bioavailability enhancement of domperidone. J Liposome Res 2022; 32(2): 172-80.
[PMID: 33944662]
[24]
Vu HTH, Hook SM, Siqueira SD, Müllertz A, Rades T, McDowell A. Are phytosomes a superior nanodelivery system for the antioxidant rutin? Int J Pharm 2018; 548(1): 82-91.
[http://dx.doi.org/10.1016/j.ijpharm.2018.06.042] [PMID: 29933062]
[25]
Direito R, Reis C, Roque L, et al. Phytosomes with persimmon (Diospyros kaki L.) extract: Preparation and preliminary demonstration of in vivo tolerability. Pharmaceutics 2019; 11(6): 296.
[http://dx.doi.org/10.3390/pharmaceutics11060296] [PMID: 31234548]
[26]
Dipierro F, Khan A, Bertuccioli A, et al. Quercetin phytosome® as a potential candidate for managing COVID-19. Minerva Gastroenterol 2021; 67(2): 19-195.
[27]
Maharjan RS, Singh AV, Hanif J, et al. Investigation of the associations between a nanomaterial’s microrheology and toxicology. ACS Omega 2022; 7(16): 13985-97.
[http://dx.doi.org/10.1021/acsomega.2c00472] [PMID: 35559161]
[28]
Sudhakar P, Kothai R, Thenmozhi V. Development of an animal model for polycystic ovarian syndrome in relation to reproductive and metabolic phenotype. J Pharm Sci Res 2019; 11(8): 2827-32.
[29]
Rondanelli M, Riva A, Petrangolini G, et al. Berberine phospholipid is an effective insulin sensitizer and improves metabolic and hormonal disorders in women with polycystic ovary syndrome: A one-group pretest–post-test explanatory study. Nutrients 2021; 13(10): 3665.
[http://dx.doi.org/10.3390/nu13103665] [PMID: 34684666]
[30]
Hebbar S, Mathias AC. Phytosomes: A novel molecular nano complex between phytomolecule and phospholipid as a value added herbal drug delivery system. Artic Int J Pharm Sci Rev Res 2018; 51(1): 84-90.
[31]
Burger AM, Mengs U, Kelter G, Schüler JB, Fiebig HH. No evidence of stimulation of human tumor cell proliferation by a standardized aqueous mistletoe extract in vitro. Anticancer Res 2003; 23(5 A): 3801-6.
[32]
OECD. Guidance document on acute oral toxicity testing. ENV/JM/MONO 2001; 4: 1-24. Available from: https://www.oecd.org/officialdocuments/publicdisplaydocumentpdf/?cote=env/jm/mono(2001)4&doclanguage=en
[33]
Jazani AM, Azgomi HND, Azgomi AND, Azgomi RND. A comprehensive review of clinical studies with herbal medicine on polycystic ovary syndrome (PCOS). Daru 2019; 27(2): 863-77.
[http://dx.doi.org/10.1007/s40199-019-00312-0] [PMID: 31741280]
[34]
OECD. Oecd guidelines for the testing of chemicals 2008. Available from: https://www.oecd-ilibrary.org/environment/oecd-guidelines-for-the-testing-of-chemicals-section-4-health-effects_20745788
[35]
Tiwari R, Tiwari G, Singh R. Allopurinol loaded transferosomes for the alleviation of symptomatic after-effects of gout: An account of pharmaceutical implications. Curr Drug Ther 2020; 15(4): 404-19.
[http://dx.doi.org/10.2174/1574885515666200120124214]
[36]
Azeez NA, Deepa VS, Sivapriya V. Phytosomes: Emergent promising nano vesicular drug delivery system for targeted tumor therapy. Adv Nat Sci 2018; 9(3): 033001.
[http://dx.doi.org/10.1088/2043-6254/aadc50]
[37]
Nandhini S, Ilango K. Development and characterization of a nano-drug delivery system containing vasaka phospholipid complex to improve bioavailability using quality by design approach. Res Pharm Sci 2020; 16(1): 103-17.
[PMID: 33953779]
[38]
Londonkar RL. Polycystic ovary syndrome (PCOS) - a mini review. Open Access J Gynecol 2018; 3(1)
[http://dx.doi.org/10.23880/OAJG-16000148]
[39]
Kamble AV, Dhamane SP, Kulkarni AS, Potnis VV. Review on effects of herbal extract for the treatment of Polycystic Ovarian Syndrome (PCOS). Plant Arch 2020; 20(1): 1189-95.
[40]
Pachiappan S, Muthusamy G, Saravanan PP, Matheswaran S. Medicinal plants for polycystic ovary syndrome: A review of phytomedicine research. Int J Herb Med 2017; 5(2): 78-80.
[41]
Rohini J, Ezzudin MR, Rabeta MS. An attempt to improve the solubility and stability of Ocimum tenuiflorum aqueous leaves extract. Food Res 2021; 5(1): 357-65.
[http://dx.doi.org/10.26656/fr.2017.5(1).425]

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