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Current Drug Research Reviews


ISSN (Print): 2589-9775
ISSN (Online): 2589-9783

Review Article

Toxicological Analysis of Drugs in Human Mummified Bodies and Proposed Guidelines

Author(s): Angela Silva-Bessa*, Shari L. Forbes, Maria Teresa Ferreira and Ricardo Jorge Dinis-Oliveira*

Volume 15, Issue 1, 2023

Published on: 10 November, 2022

Page: [62 - 72] Pages: 11

DOI: 10.2174/2589977514666220914084543

Price: $65


From palaeopathology to forensic taphonomy, mummified human bodies constitute biological archives of paramount importance. Toxicology analysis of endobiotics and xenobiotics has already shown value to archaeological mummies research with detecting heavy metals, sedative-hypnotic drugs, and stimulants. Thanks to the large window of drug detection in hair and nails, the information from such studies has increased the scientific community’s knowledge regarding past populations’ lifestyles. Still, few bibliographic references exist regarding toxicology reports in mummified bodies from forensic settings. Here, the authors aim to draw attention to the valuable contribution of toxicology analysis, taking into account previously conducted studies and their findings. Given that sample collection on mummified bodies from forensic scenarios may not always happen in laboratories or autopsy rooms, the authors also suggest guidelines for in situ sampling of forensic mummies. It is expected that the present technical note will encourage experts to perform toxicology analysis in mummified bodies and publish their case reports more often.

Keywords: Mummy, mummification, toxicology analysis, sample collection, palaeopathology, forensic taphonomy, endobiotics.

Graphical Abstract
Ikram S. In: Wendrich W, Ed..UCLA Encyclopedia of Egyptology. Los Angeles: UCLA 2010; pp. 1-5.
Aufderheide AC. The scientific study of mummies. Cambridge: Cambridge University Press 2010.
Lynnerup N. Mummies. Am J Phys Anthropol 2007; 134: 162-90.
Pinheiro J. In: Schmitt A, Cunha E, Pinheiro J, Eds.a cadaver. Forensic anthropology and medicine: Complementary sciences from recovery to cause of death. Washington: Humana Press 2006; pp. 85-116.
Nystrom KC. The bioarchaeology of mummies. New York: Routledge 2018.
Piombino-Mascali D. Mummy stories. Archaeol Litu 2019; 20: 139-50.
Nerlich AG, Bianucci R. In: Shin DH, Bianucci R, Eds. The Handbook of Mummy Studies. Singapore: Springer 2020; pp. 741-776.
Slepchenko SM, Novikov IK, Hong JH, et al. In: Shin DH, Bianucci R, Eds. The handbook of mummy studies. Singapore: Springer 2020; pp. 833-850.
Beckett RG. In: Shin DH, Bianucci R, Eds. The Handbook of Mummy Studies. Singapore: Springer 2020; pp. 957-982.
Arriaza BT. Arseniasis as an environmental hypothetical explanation for the origin of the oldest artificial mummification practice in the world. Chungara (Arica) 2005; 37(2): 255-60.
Jones J, Higham TFG, Oldfield R, O’Connor TP, Buckley SA. Evidence for prehistoric origins of Egyptian mummification in late Neolithic burials. PLoS One 2014; 9(8): e103608.
Jackowski C, Bolliger S, Thali MJ. Common and unexpected findings in mummies from ancient Egypt and South America as revealed by CT. Radiographics 2008; 28(5): 1477-92.
Abdel-Maksoud G, El-Amin A-R. A review on the materials used during the mummification processes in ancient Egypt. Mediterr Archaeol Archaeom Int J 2011; 11: 129-50.
Alterauge AS, Hofmann C. Crypt burials from the cloister church of riesa (Germany)-changes of funerary customs, body treatment, and attitudes to death. Acta Universitatis Lodziensis Folia Archaeologica 2019; 35: 73-96.
Hashesh Z, Herrerín J. Dental Prosthesis: postmortem treatment of oral reconstruction during the mummification process. J Am Res Cent Egypt 2019; 55: 55-67.
Barnes KM, Whiffin AL, Bulling MT. A preliminary study on the antibacterial activity and insect repellent properties of embalming fluids from the 18th Dynasty (1550–1292 BCE) in ancient Egypt. J Archaeol Sci Rep 2019; 25: 600-9.
Finaughty DA, Morris AG. Precocious natural mummification in a temperate climate (Western Cape, South Africa). Forensic Sci Int 2019; 303: 109948.
Piombino-Mascali D, Nystrom KC. In: Betsinger TK, Scott AB, Tsaliki A, Eds. The Odd, the unusual, and the strange: bioarchaeological explorations of atypical burials. 1st ed. Florida: University Press of Florida 2019; pp. 312-22.
Bhise SS, Nanandkar SD, Thube HR. Mixed picture of decomposition, adipocere & mummification well preserved the ligature mark in hanging body: A case report. J Forensic Leg Med Sci Law 2011; 20: 60-6.
König W. Photographien von Röntgen-Strahlen aufgenommen im physikalischen Verein zu Frankfurt AM. Leipzig: Johan Ambrosius Barth 1986.
Harwood-Nash DCF. Computed tomography of ancient Egyptian mummies. J Comput Assist Tomogr 1979; 3(6): 768-73.
Olympus Corporation [homepage on the Internet]. History of Olympus products: endoscopes [cited 2022 Feb 9]. Available from:
Manialawi M, Meligy R, Bucaille M. Endoscopic examination of Egyptian mummies. Endoscopy 1978; 10(3): 191-4.
Yatsishina EB, Pozhidaev VM, Sergeeva YE, Malakhov SN, Slushnaya IS. An integrated study of the hair coating of ancient Egyptian mummies. J Anal Chem 2020; 75(2): 262-74.
Egeland GM, Ponce R, Bloom NS, et al. Hair methylmercury levels of mummies of the Aleutian Islands, Alaska. Environ Res 2009; 109(3): 281-6.
Blumenstiel D, McDonald M, Arriaza B, Amarasiriwardena D. Exposure to geogenic lithium in ancient Andeans: Unraveling lithium in mummy hair using LA-ICP-MS. J Archaeol Sci 2020; 113: 105062.
Mari F, Polettini A, Lippi D, Bertol E. The mysterious death of Francesco I de’ Medici and Bianca Cappello: an arsenic murder? BMJ 2006; 333(7582): 1299-301.
Fresnais M, Richardin P, Gimat A, Sepúlveda M, Leize-Wagner E, Charrié A. Recent advances in the characterization of hair of mummies from the Chilean Andean coast. Forensic Sci Int 2015; 249: 25-34.
Idrovo AJ, Romero WM, Silva E, Villamil de García G, Ortiz JE. Mercury determination in prehispanic Colombian biological samples: First experiences and investigation perspectives. Biomedica 2002; 22: 67-70.
Arriaza B, Blumenstiel D, Amarasiriwardena D, Standen VG, Vizcarra A. Five thousand years of bellyaches: Exploring boron concentration in ancient populations of the Atacama Desert. Am J Phys Anthropol 2021; 174(2): 254-67.
Apostoli P, De Palma G, Catalani S, Bortolotti F, Tagliaro F. Multielemental analysis of tissues from Cangrande della Scala, Prince of Verona, in the 14th century. J Anal Toxicol 2009; 33(6): 322-7.
Fornaciari G, Marinozzi S, Gazzaniga V, et al. The use of mercury against pediculosis in the Renaissance: The case of Ferdinand II of Aragon, King of Naples, 1467-96. Med Hist 2011; 55(1): 109-15.
Hatje V, Lamborg CH, Boyle EA. Trace-metal contaminants: Human footprint on the ocean. Elements 2018; 14(6): 403-8.
Wang W-X, Rainbow PS. Environmental Pollution of the Pearl River Estuary, China. Berlin: Springer 2020.
Branco V, Caito S, Farina M, Teixeira da Rocha J, Aschner M, Carvalho C. Biomarkers of mercury toxicity: Past, present, and future trends. J Toxicol Environ Health B Crit Rev 2017; 20(3): 119-54.
Kawahata H, Yamashita S, Yamaoka K, Okai T, Shimoda G, Imai N. Heavy metal pollution in Ancient Nara, Japan, during the eighth century. Prog Earth Planet Sci 2014; 1(1): 15.
MacLeod-Glover N, Chuang R. Chronic lithium toxicity: Considerations and systems analysis. Can Fam Physician 2020; 66(4): 258-61.
Waring WS. Management of lithium toxicity. Toxicol Rev 2006; 25(4): 221-30.
Mimica N, Prejac J, Skalny AA, Grabeklis AR. Momčilović1 B. Assessing lithium nutritional status by analyzing its cumulative frequency distribution in the hair and whole blood. Trace Elem Electrolytes 2019; 36: 113-9.
Mischley L. The role of lithium in neurological health and disease. J Orthomol Med 2014; 29: 101-8.
Prejac J, Skalny AA, Grabeklis AR, Uzun S, Mimica N. Momčilović B. Assessing the boron nutritional status by analyzing its cummulative frequency distribution in the hair and whole blood. J Trace Elem Med Biol 2018; 45: 50-6.
Soriano-Ursúa MA, Farfán-García ED, Geninatti-Crich S. Turning fear of boron toxicity into boron-containing drug design. Curr Med Chem 2019; 26(26): 5005-18.
Gallello G, Cilli E, Bartoli F, et al. Poisoning histories in the Italian renaissance: The case of Pico Della Mirandola and Angelo Poliziano. J Forensic Leg Med 2018; 56: 83-9.
Ratnaike RN. Acute and chronic arsenic toxicity. Postgrad Med J 2003; 79(933): 391-6.
Jomova K, Jenisova Z, Feszterova M, et al. Arsenic: Toxicity, oxidative stress and human disease. J Appl Toxicol 2011; 31(2): n/a.
Dinis-Oliveira RJ, Vieira DN, Magalhães T. Guidelines for collection of biological samples for clinical and forensic toxicological analysis. Forensic Sci Res 2016; 1(1): 42-51.
Fornaciari A, Gaeta R, Chericoni S, et al. Cancer and therapy in the 16th century: The unique case of adenocarcinoma in Luigi Carafa, prince of Stigliano (1511–76). Lancet Oncol 2019; 20(12): 1641-2.
Charlier P, Huynh-Charlier I, Poupon J, et al. The heart of blessed Anne-Madeleine Remuzat: A biomedical approach of “miraculous” heart conservation. Cardiovasc Pathol 2014; 23(6): 344-50.
Charlier P, Poupon J, Jeannel GF, et al. The embalming of John of Lancaster, first Duke of Bedford (1435 AD): A forensic analysis. Med Sci Law 2016; 56(2): 107-15.
Charlier P, Poupon J, Jeannel GF, et al. The embalmed heart of Richard the Lionheart (1199 A.D.): A biological and anthropological analysis. Sci Rep 2013; 3(1): 1296.
Corbineau R, Ruas MP, Barbier-Pain D, Fornaciari G, Dupont H, Colleter R. Plants and aromatics for embalming in Late Middle Ages and modern period: A synthesis of written sources and archaeobotanical data (France, Italy). Veg Hist Archaeobot 2018; 27(1): 151-64.
Musshoff F, Brockmann C, Madea B, Rosendahl W, Piombino-Mascali D. Ethyl glucuronide findings in hair samples from the mummies of the Capuchin Catacombs of Palermo. Forensic Sci Int 2013; 232(1-3): 213-7.
Balabanova S, Parsche S, Pirsig W. First identification of drugs in Egyptian mummies. Naturwissenschaften 1992; 79(8): 358.
Parsche F, Balabanova S, Pirsig W. Drugs in ancient populations. Lancet 1993; 341(8843): 503.
Springfield AC, Cartmell LW, Aufderheide AC, Buikstra J, Ho J. Cocaine and metabolites in the hair of ancient Peruvian coca leaf chewers. Forensic Sci Int 1993; 63(1-3): 269-75.
Ogalde JP, Arriaza BT, Soto EC. Identification of psychoactive alkaloids in ancient Andean human hair by gas chromatography/mass spectrometry. J Archaeol Sci 2009; 36(2): 467-72.
Musshoff F, Rosendahl W, Madea B. Determination of nicotine in hair samples of pre-Columbian mummies. Forensic Sci Int 2009; 185(1-3): 84-8.
Musshoff F, Fels H, Carli A, Piombino-Mascali D. The anatomical mummies of Mombello: Detection of cocaine, nicotine, and caffeine in the hair of psychiatric patients of the early 20th century. Forensic Sci Int 2017; 270: 20-4.
Cartmell LW, Aufderhide A, Weems C. Cocaine metabolites in pre-Columbian mummy hair. J Okla State Med Assoc 1991; 84(1): 11-2.
Liu L, Wang J, Rosenberg D, Zhao H, Lengyel G, Nadel D. Fermented beverage and food storage in 13,000 y-old stone mortars at Raqefet Cave, Israel: Investigating Natufian ritual feasting. J Archaeol Sci Rep 2018; 21: 783-93.
Hanson DJ. In: Boyle P, Boffetta P, Lowenfels AB, Burns H, Brawley O, Zatonski W, Rehm J, Eds. Alcohol: Science, policy and public health. Oxford: Oxford University Press 2013; pp. 56-80.
Gately I. Drink: A cultural history of alcohol. New York: Gotham Books 2008.
Nahas GG. Hashish in Islam 9th to 18th century. Bull N Y Acad Med 1982; 58(9): 814-31.
Ghiabi M, Maarefvand M, Bahari H, Alavi Z. Islam and cannabis: Legalisation and religious debate in Iran. Int J Drug Policy 2018; 56: 121-7.
Baasher T. The use of drugs in the Islamic world. Addiction 1981; 76(3): 233-43.
Cartmell LW, Aufderheide AC, Springfield A, Weems C, Arriaza B. The frequency and antiquity of prehistoric coca-leaf-chewing practices in northern Chile: Radioimmunoassay of a cocaine metabolite in human-mummy hair. Lat Am Antiq 1991; 2(3): 260-8.
Hedegaard H, Miniño AM, Warner M. Drug overdose deaths in the United States, 1999-2018 2020.
McCall Jones C, Baldwin GT, Compton WM. Recent increases in cocaine-related overdose deaths and the role of opioids. Am J Public Health 2017; 107(3): 430-2.
Taghaddosinejad F, Arefi M, Fayaz AF, Tanhaeivash R. Determination of substance overdose in two Iranian centers: Comparison between opioids and non-opioids. J Forensic Leg Med 2013; 20(3): 155-7.
Rosenblum D, Unick J, Ciccarone D. The rapidly changing US illicit drug market and the potential for an improved early warning system: Evidence from Ohio drug crime labs. Drug Alcohol Depend 2020; 208: 107779.
Quadra GR, Paranaíba JR, Vilas-Boas J, et al. A global trend of caffeine consumption over time and related-environmental impacts. Environ Pollut 2020; 256: 113343.
Diogo JSG, Silva LSO, Pena A, Lino CM. Risk assessment of additives through soft drinks and nectars consumption on Portuguese population: A 2010 survey. Food Chem Toxicol 2013; 62: 548-53.
Sharapova S, Reyes-Guzman C, Singh T, Phillips E, Marynak KL, Agaku I. Age of tobacco use initiation and association with current use and nicotine dependence among US middle and high school students, 2014–2016. Tob Control 2020; 29(1): 49-54.
Chinwong D, Mookmanee N, Chongpornchai J, Chinwong S. A comparison of gender differences in smoking behaviors, intention to quit, and nicotine dependence among Thai university students. J Addict 2018 2018.
dos Santos RG. The pharmacology of ayahuasca: A review. Brasília Médica 2010; 47: 188-95.
Antunes HF. Drugs, religion, and cultural heritage: An analysis of the public policies regarding the use of Ayahuasca in Brazil. Int J Religion Spiritual Soc 2019; 9(1): 29-39.
Brito-da-Costa AM, Dias-da-Silva D, Gomes NGM, Dinis-Oliveira RJ, Madureira-Carvalho Á. Toxicokinetics and toxicodynamics of ayahuasca alkaloids N,N-Dimethyltryptamine (DMT), harmine, harmaline and tetrahydroharmine: Clinical and forensic impact. Pharmaceuticals (Basel) 2020; 13(11): 334.
Murdock J. [homepage on the Internet]. Mummified body of elderly man found in apartment 11 years after he died. 2019.[cited 2021 Nov 24]. Available from:
Gander K. [homepage on the Internet]. Schoolboy finds mummified body while exploring abandoned house. Independent 2014. [cited 2021 Nov 24]. Available from:
Graffius C. [homepage on the Internet]. Body of man, 28, found "mummified" as he lay dead for nine months after worrying about claiming benefits. The Sun 2020. [cited 2021 Nov 24]. Available from:
Fonrouge G. [homepage on the Internet]. Stunning photos show home where hoarder's mummified body was found. New York Post 2021. [cited 2021 Nov 24]. Available from:
Golgowski N. Mummified body of explorer found sitting at desk on drifting yacht. Huffington Post 2016.
Warrander R. Patterson S. [homepage on the Internet]. Body in sheets: Fears Scots OAP "mummified" wife's corpse in duvet & kept remains in house for 12 years. The Scottish Sun 2021. [cited 2021 Nov 24]. Available from:
Blankstein A. [homepage on the Internet]. Mummified body of former playboy playmate Yvette vickers found in her Benedict Canyon home. Los Angeles Times 2011. [cited 2021 Nov 24]. Available from:
Hönigschnabl S, Schaden E, Stichenwirth M, et al. Discovery of decomposed and mummified corpses in the domestic setting-a marker of social isolation? J Forensic Sci 2002; 47(4): 15453J.
den Hondel KE, Buster M, Bender P, Reijnders U. Ten-year-old mummified corpse found in domestic setting in Rotterdam the Netherlands. J Forensics Res 2016; 7(3): 2.
De Dominicis E, Arcudi G, Mauriello S, Marsella LT. Spontaneous corification: A case of anomalous decomposition. Minerva Medicoleg 2017; 137: 48-51.
Kadej M. Szleszkowski Ł Thannhäuser A, Jurek T. A mummified human corpse and associated insects of forensic importance in indoor conditions. Int J Legal Med 2020; 134(5): 1963-71.
Ferreira MT, Cunha E. Será credível estimar o PMI em restos cadavéricos em avançado estado de decomposição? Cadernos do GEEvH 2012; 2: 7-20.
Campobasso CP, Falamingo R, Grattagliano I, Vinci F. The mummified corpse in a domestic setting. Am J Forensic Med Pathol 2009; 30(3): 307-10.
Catanese G, Bloom T. Recovery of a mummified pregnant woman from a 55-gallon drum more than 30 years after her death. Am J Forensic Med Pathol 2002; 23(3): 245-7.
Jit I, Sehgal S, Sahni D. An Indian mummy: A case report. Forensic Sci Int 2001; 117(1-2): 57-63.
Ventura F, Portunato F, Pizzorno E, Mazzone S, Verde A, Rocca G. The need for an interdisciplinary approach in forensic sciences: Perspectives from a peculiar case of mummification. J Forensic Sci 2013; 58(3): 831-6.
Catanesi R, Punzi G, Rodriguez WC III, Solarino B, Di Vella G. Faith, folie à famille, and mummification: A brief review of the literature and a rare case report. J Forensic Sci 2014; 59(1): 274-80.
Gitto L, Bonaccorso L, Maiese A, dell’Aquila M, Arena V, Bolino G. A scream from the past: A multidisciplinary approach in a concealment of a corpse found mummified. J Forensic Leg Med 2015; 32: 53-8.
Politi L, Morini L, Mari F, Groppi A, Bertol E. Ethyl glucuronide and ethyl sulfate in autopsy samples 27 years after death. Int J Legal Med 2008; 122(6): 507-9.
Giusiani M, Chericoni S, Domenici R. Identification and quantification of phenobarbital in a mummified body 10 years after death. J Forensic Sci 2012; 57(5): 1384-7.
Harding BE, Sullivan LM, Adams S, Middleberg RA, Wolf BC. Multidisciplinary investigation of an unusual apparent homicide/suicide. Am J Forensic Med Pathol 2011; 32(3): 208-12.
Ortmann C, DuChesne A. A partially mummified corpse with pink teeth and pink nails. Int J Legal Med 1997; 111(1): 35-7.
Wegner A, Doberentz E, Madea B. Death in the sauna-vitality markers for heat exposure. Int J Legal Med 2021; 135(3): 903-8.
Hayashi T, Buschmann C, Tsokos M. Complete post-mortem decapitation in suicidal hanging. Forensic Sci Med Pathol 2012; 8(4): 463-5.
Hänsch C. An unusual case of body mummification. Z Rechtsmed 1974; 75(3): 225-7.
Wills SM, Johnson CP. Homicidal smothering: Vital histological confirmation of orofacial injury despite a prolonged post-mortem interval. Forensic Sci Med Pathol 2009; 5(1): 28-31.
Kudo K, Sugie H, Syoui N, et al. Detection of triazolam in skeletal remains buried for 4 years. Int J Legal Med 1997; 110(5): 281-3.
Galtés I, Gallego MÁ, Giménez D, et al. A body, a dog, and a fistful of scats. Forensic Sci Int 2014; 241: e1-4.
Introna F, De Donno A, Santoro V, et al. The bodies of two missing children in an enclosed underground environment. Forensic Sci Int 2011; 207(1-3): e40-7.
Marella GL, Perfetti E, Manciocchi S, Arcudi G. A case of “precocious” mummification. J Forensic Leg Med 2013; 20(2): 122-4.
Usui A, Kawasumi Y, Hosokai Y, Hayashizaki Y, Saito H, Funayama M. Radiological analysis of a naturally mummified body. Jpn J Radiol 2012; 30(5): 458-62.
Dedouit F, Guilbeau-Frugier C, Telmon N, et al. Virtual autopsy and forensic anthropology of a mummified fetus: A report of one case. J Forensic Sci 2008; 53(1): 208-12.
Loreille OM, Parr RL, McGregor KA, et al. Integrated DNA and fingerprint analyses in the identification of 60-year-old mummified human remains discovered in an Alaskan glacier. J Forensic Sci 2010; 55(3): 813-8.
Beaumont G. Body on the beach… a case of foot and mouth? J Forensic Leg Med 2007; 14(1): 54-7.
Cortellini V, Brescia G, Simoes Dutra Correa H, Cerri N, Bin P, Verzeletti A. Living with the dead: A case report and review of the literature. J Forensic Leg Med 2019; 62: 107-11.
Idota N, Nakamura M, Masui K, Kakiuchi Y, Yamada K, Ikegaya H. Lessons Learned from Autopsying an Unidentified Body with Iodine-125 Seeds Implanted for Prostate Brachytherapy. J Forensic Sci 2017; 62(2): 536-40.
Kemp W, McKeown A, Symes S, Skelton R. Identification of skeletal fractures before and after removal of soft tissue: A case report. Am J Forensic Med Pathol 2013; 34(1): 18-22.
Harding BE, Wolf BC. The phenomenon of the urban mummy. J Forensic Sci 2015; 60(6): 1654-7.
Alterauge A, Kellinghaus M, Jackowski C, et al. The Sommersdorf mummies-An interdisciplinary investigation on human remains from a 17th-19th century aristocratic crypt in southern Germany. PLoS One 2017; 12(8): e0183588.
Silva-Bessa A, Forbes SL, Dinis-Oliveira RJ, Madureira-Carvalho Á, Ferreira MT. A preliminary study on five exhumed bodies and their burial graves from the Cemetery of Prado do Repouso (Porto, Portugal). RevSALUS-Revista Científica Internacional da Rede Académica das Ciências da Saúde da Lusofonia 2022; 4: 25-5.
Spongberg AL, Becks PM. Inorganic soil contamination from cemetery leachate. Water Air Soil Pollut 2000; 117(1/4): 313-27.
Dinis-Oliveira RJ, Carvalho F, Duarte JA, et al. Collection of biological samples in forensic toxicology. Toxicol Mech Methods 2010; 20(7): 363-414.
Barbosa J, Faria J, Carvalho F, et al. Hair as an alternative matrix in bioanalysis. Bioanalysis 2013; 5(8): 895-914.
Klima M, Altenburger MJ, Kempf J, Auwärter V, Neukamm MA. Determination of medicinal and illicit drugs in post mortem dental hard tissues and comparison with analytical results for body fluids and hair samples. Forensic Sci Int 2016; 265: 166-71.

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