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Combinatorial Chemistry & High Throughput Screening

Editor-in-Chief

ISSN (Print): 1386-2073
ISSN (Online): 1875-5402

Cell-Based Assays in Practice: Cell Markers from Autofluorescent Proteins of the GFP-Family

Author(s): Michael Wolff, Simone Kredel, Jorg Wiedenmann, G. Ulrich Nienhaus and Ralf Heilker

Volume 11 , Issue 8 , 2008

Page: [602 - 609] Pages: 8

DOI: 10.2174/138620708785739880

Price: $65

Abstract

The more recently discovered anthozoan fluorescent proteins (FPs) and the classic Aequorea victoria Green Fluorescent Protein (avGFP) as well as their derivatives have become versatile tools as live cell markers in fluorescence microscopy. In this review, we show the use of these FPs in drug discovery assays. Assay examples are given for the application of FPs in multiplexed imaging, as photosensitizers, as fluorescent timers, as pulse-chase labels and for robotically integrated compound testing. The development of fast microscopic imaging devices has enabled the application of automated fluorescence microscopy combined with image analysis to pharmaceutical high throughput drug discovery assays, generally referred to as High Content Screening (HCS).

Keywords: Fluorescent proteins, High Content Screening, fluorescence microscopy, imaging, anthozoan


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