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Current Drug Delivery

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ISSN (Print): 1567-2018
ISSN (Online): 1875-5704

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Research Article

Development of a Novel Pomegranate Polysaccharide Nanoemulsion Formulation with Anti-Inflammatory, Antioxidant, and Antitumor Properties

Author(s): Samah S. Hoseny, Amel M. Soliman, Sohair R. Fahmy and Shimaa A. Sadek*

Volume 20, Issue 5, 2023

Published on: 22 July, 2022

Page: [575 - 586] Pages: 12

DOI: 10.2174/1567201819666220509161548

Price: $65

Abstract

Background: Colorectal cancer is one of the most serious gastrointestinal cancers in Africa and its prevention is a pronounced challenge in contemporary medicine worldwide.

Objective: The present study aimed to develop nanoemulsion drug delivery system using pomegranate polysaccharides (PGPs) as an alternative cancer remedy, and then the evaluated its biological activities.

Methods: The PGPs yield and chemical composition were evaluated, and then a PGPs nanoemulsion (PGPs-NE) was prepared using the self-emulsification technique with an oil phase. The physicochemical characterization of PGPs-NE was then analyzed. The in vitro antioxidant, anti-inflammatory activities, and antitumor potency of PGPs and PGPs-NE were also evaluated.

Results: The PGPs yield was 10%. The total sugar and protein content of PGPs was 44.66 mg/dl and 19.83μg/ml, respectively. PGPs were mainly composed of five monosaccharides including fructose, glucose, galactose, rhamnose, and arabinose. Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204, and 207nm and a transmittance of 80%. Its average hydrodynamic particle size was 9.5nm, with a PDI of less than 0.2 and a negative zeta potential (-30.6 mV). The spherical shape of PGPs-NE was confirmed by a transmission electron microscope study, with an average size of less than 50 nm. Additionally, the method used to prepare the PGPs-NE formulation provided high entrapment efficiency (92.82%). The current study disclosed that PGPs-NE exhibited strong antioxidant, anti-inflammatory, and antitumor agent potency compared to that of free PGPs.

Conclusion: These promising current findings provide evidence for the possible efficacy of novel PGPs-NE as an alternative treatment for CRC.

Keywords: Colorectal cancer, drug delivery system, nanoemulsion, pomegranate polysaccharides, antioxidant, antiinflammatory.

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