Impact on Growth and Secondary Metabolites in White Poplar (Populus alba L.) Callus using SeNPs@Moringa

Title:Impact on Growth and Secondary Metabolites in White Poplar (Populus alba L.) Callus using SeNPs@Moringa

Volume: 15 Issue: 2

Author(s): Reda E. Abo El-Fadl, Mahmoud M. El-Saber , Manal E.A. Ahmed, Tamer M. Abd Elaziem and Ahmed A. El-Sayed*

Affiliation:

• Department of Photochemistry, National Research Centre, 33 El-Bohouth St., Dokki-12622, Giza, Egypt

Keywords: White poplar, callus SeNPs, green nanotechnology, secondary metabolite, GCMS, DPPH.

Abstract:

Background: Green synthesis of nanoparticles has provided a cost-effective, environmentally friendly method, as well as raised safe strategies for the synthesis of nanomaterials.

Methods: In this study, we investigated the synthesis of Selenium nanoparticles from the leaf aqueous extract of Moringa oleifera (MO-SeNPs) and studied the effect of MO-SeNPs and Moringa extract with various concentrations on the growth, active constituents, and antioxidant activity (scavenging DPPH) of Populus alba callus. MS medium supplemented with (2, 4-D at 2 mg/l + 0.5 mg/l BA) took the maximum callus induction percentage (100%), increasing the callus fresh weight, dry weight, and degree of callus formation compared with (2.0 mg/l 2,4-D + 0.5 mg/l Kin).

Results: The highest values of callus fresh and dry weight were recorded on MS containing 20% MO-SeNPs. Regarding the active constituents, the GC-MS chromatogram revealed the presence of 23 major compounds identified in the chloroform extract. The phytoconstituents with maximum peak area in callus extract are Tetradecane, Hexadecen-1-ol, Trans-9, 5-Octadecene, (Benzene, 1- pentyloctyl), (Benzene, 1-butylnonyl), Cycloeicosane, and 10-Heneicosene in all treatments. Moringa extract and MO-SeNPs stimulate the increase of compounds in callus compared to control. In this regard, MO-SeNPs 5% led to a higher increase in the area percent of active substances compared to the treatment of Moringa extract and control. Concerning the antioxidant activity, the results indicated that Moringa extract and MO-SeNPs had a positive effect on scavenging DPPH compared with the control. The highest percentages of scavenging DPPH were recorded when Populus alba L. callus treated with MO-SeNPs 20%, 10%, and Moringa extract 20%, percentages reached (84.65, 75.35, and 71.94%), respectively.

Conclusion: Green synthesis of MO-SeNPs improved secondary metabolites and antioxidant activity in Populus alba L. callus.

Cite this article as:

Abo El-Fadl E. Reda , El-Saber M. Mahmoud , Ahmed E.A. Manal, Abd Elaziem M. Tamer and El-Sayed A. Ahmed*, Impact on Growth and Secondary Metabolites in White Poplar (Populus alba L.) Callus using SeNPs@Moringa, Current Materials Science 2022; 15 (2) . https://dx.doi.org/10.2174/2666145414666211125093640