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Protein & Peptide Letters

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ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

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Research Article

Exploring the Molecular Level Interaction of Human Serum Albumin with Calcium Oxalate Monohydrate Crystals

Author(s): Priyadarshini*, Abhishek Negi, Chetna Faujdar, Lokesh Nigam and Naidu Subbarao

Volume 28, Issue 11, 2021

Published on: 30 September, 2021

Page: [1281 - 1289] Pages: 9

DOI: 10.2174/0929866528666210930165426

Price: $65

Abstract

Background: Human serum albumin (HSA) is one of the most abundant proteins in the blood plasma, urine as well as in the organic matrix of renal calculi. Macromolecules present in the urine modulate kidney stone formation either by stimulating or inhibiting the crystallization process.

Objective: In the present study, the effect of HSA protein on the growth of calcium oxalate monohydrate crystal (COM) was investigated.

Methods: Crystal growth assay was used to measure oxalate depletion in the crystal seeded solution in the presence of HSA. HSA concentrations exhibiting effect on crystal growth were selected for FTIR and XRD analysis. In silico docking was performed on seven different binding sites of HSA.

Results: Albumin plays dual role in the growth of calcium oxalate crystallization. FTIR and XRD studies further revealed HSA exerted strain over crystal thus affecting its structure by interacting with amino acids of its pocket 1. Docking results indicate that out of 7 binding pocket in protein, calcium oxalate interacts with Arg-186 and Lys-190 amino acids of pocket 1.

Conclusion: Our study confirms the role of HSA in calcium oxalate crystallization where acidic amino acids arginine and lysine bind to COM crystals, revealing molecular interaction of macromolecule and crystal in urolithiasis.

Keywords: Human serum albumin, calcium oxalate monohydrate crystals, molecular docking, crystallization, X-ray diffraction, fourier-transform infrared spectroscopy.

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